Anti-ISG20 antibody (ab50834)


  • Product name
  • Description
    Rabbit polyclonal to ISG20
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: VLYDKFIRPE GEITDYRTRV SGVTPQHMVG ATPFAVARLE ILQLLKGKLV , corresponding to amino acids 37-86 of Human ISG20

  • Positive control
    • Daudi Cell Lysate



Our Abpromise guarantee covers the use of ab50834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay is 1:1562500.

IHC-P Use at an assay dependent concentration.


  • Function
    Exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. Degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. Involved in the antiviral function of IFN against RNA viruses.
  • Tissue specificity
    Highly expressed in peripheral blood leukocytes, spleen, thymus, colon and lung.
  • Sequence similarities
    Belongs to the exonuclease superfamily.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CD25 antibody
    • Estrogen regulated transcript 45 protein antibody
    • Estrogen-regulated transcript 45 protein antibody
    • HEM45 antibody
    • Interferon stimulated exonuclease gene 20kDa antibody
    • Interferon stimulated gene 20 kDa protein antibody
    • Interferon-stimulated gene 20 kDa protein antibody
    • Isg20 antibody
    • ISG20_HUMAN antibody
    • Promyelocytic leukemia nuclear body associated protein antibody
    • Promyelocytic leukemia nuclear body-associated protein ISG20 antibody
    see all

Anti-ISG20 antibody images

  • Anti-ISG20 antibody (ab50834) at 1.25 µg/ml + Daudi Cell Lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 50000

    Predicted band size : 20 kDa
    Observed band size : 20 kDa

References for Anti-ISG20 antibody (ab50834)

ab50834 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (Raw 264.7 cells and THP1 cells)
Gel Running Conditions
Reduced Denaturing (12% gel (Acrylamide))
Loading amount
20 µg
Interferon bete (2h, 12h, 24h)
Raw 264.7 cells and THP1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 17 2016

I am very pleased to hear you would like to accept our offer and test ab50834 in mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the A...

Read More

Thank you for contacting Abcam regarding ab50834.

We have not yet received feedback on how this antibody works in mouse. If you are interested in testing this yourself, I am happy to offer the same testing discount as this customer had re...

Read More

I am very pleased to hear you would like to accept our offer and test ab50834 in mouse. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the &l...

Read More

Thank you very much for your interest in ab50834 and ab55338.

To our knowledge, neither antibody has been tested in mouse.

We do not have trial sizes of our products. Therefore, I can offer a discount off a future purcha...

Read More


Sign up