Anti-ISG20 antibody (ab50834)

Overview

  • Product nameAnti-ISG20 antibody
    See all ISG20 primary antibodies
  • Description
    Rabbit polyclonal to ISG20
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: VLYDKFIRPE GEITDYRTRV SGVTPQHMVG ATPFAVARLE ILQLLKGKLV , corresponding to amino acids 37-86 of Human ISG20

  • Positive control
    • Daudi Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay is 1:1562500.

IHC-P Use at an assay dependent concentration.

Target

  • FunctionExonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. Degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. Involved in the antiviral function of IFN against RNA viruses.
  • Tissue specificityHighly expressed in peripheral blood leukocytes, spleen, thymus, colon and lung.
  • Sequence similaritiesBelongs to the exonuclease superfamily.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD25 antibody
    • Estrogen regulated transcript 45 protein antibody
    • Estrogen-regulated transcript 45 protein antibody
    • HEM45 antibody
    • Interferon stimulated exonuclease gene 20kDa antibody
    • Interferon stimulated gene 20 kDa protein antibody
    • Interferon-stimulated gene 20 kDa protein antibody
    • Isg20 antibody
    • ISG20_HUMAN antibody
    • Promyelocytic leukemia nuclear body associated protein antibody
    • Promyelocytic leukemia nuclear body-associated protein ISG20 antibody
    see all

Anti-ISG20 antibody images

  • Anti-ISG20 antibody (ab50834) at 1.25 µg/ml + Daudi Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 50000

    Predicted band size : 20 kDa
    Observed band size : 20 kDa

References for Anti-ISG20 antibody (ab50834)

ab50834 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Raw 264.7 cells and THP1 cells)
Gel Running Conditions Reduced Denaturing (12% gel (Acrylamide))
Loading amount 20 µg
Treatment Interferon bete (2h, 12h, 24h)
Specification Raw 264.7 cells and THP1 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 17 2016

I am very pleased to hear you would like to accept our offer and test ab50834 in mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the A...

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Thank you for contacting Abcam regarding ab50834.


We have not yet received feedback on how this antibody works in mouse. If you are interested in testing this yourself, I am happy to offer the same testing discount as this customer had re...

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I am very pleased to hear you would like to accept our offer and test ab50834 in mouse. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the &l...

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Thank you very much for your interest in ab50834 and ab55338.

To our knowledge, neither antibody has been tested in mouse.



We do not have trial sizes of our products. Therefore, I can offer a discount off a future purcha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"