Recombinant
RabMAb

Anti-Islet 1 antibody [EP4182] (ab109517)

Overview

  • Product name
    Anti-Islet 1 antibody [EP4182]
    See all Islet 1 primary antibodies
  • Description
    Rabbit monoclonal [EP4182] to Islet 1
  • Specificity
    86% identities with Islet 2
  • Tested applications
    Suitable for: ICC/IF, WB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Islet 1 aa 250 to the C-terminus.
    Database link: P61371

  • Positive control
    • WB: K562, HeLa, Jurkat and SH-SY5Y cell lysates IHC-Fr: Chicken hindbrain ICC/IF: U-87 MG cell line.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Islet 1 antibody (Alexa Fluor® 594) [EP4182] (ab203404)

    Anti-Islet 1 antibody (Alexa Fluor® 555) [EP4182] (ab203405)

    Anti-Islet 1 antibody (Alexa Fluor® 405) [EP4182] (ab202297)

    Anti-Islet 1 antibody (Alexa Fluor® 568) [EP4182] (ab203406)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.

For unpurified use at 1/50 - 1/100.

WB 1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
IP 1/10 - 1/100.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Binds to one of the cis-acting domain of the insulin gene enhancer.
  • Tissue specificity
    Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
  • Sequence similarities
    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Insulin gene enhancer protein ISL 1 antibody
    • Insulin gene enhancer protein ISL-1 antibody
    • Insulin related protein antibody
    • ISL 1 antibody
    • ISL LIM homeobox 1 antibody
    • ISL1 antibody
    • ISL1 transcription factor LIM homeodomain antibody
    • ISL1 transcription factor, LIM/homeodomain (islet 1) antibody
    • ISL1 transcription factor, LIM/homeodomain antibody
    • ISL1_HUMAN antibody
    • Islet-1 antibody
    • Islet1 antibody
    see all

Anti-Islet 1 antibody [EP4182] images

  • Anti-Islet 1 antibody [EP4182] (ab109517) at 1/50000 dilution (purified) + RAW264.7 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 39 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Flow Cytometry analysis of SH-SY5Y(human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Anti-Islet 1 antibody [EP4182] (ab109517) at 1/10000 dilution (purified) + UMNSAH/DF-1 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 39 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • IHC-Fr image of anti-Islet 1 staining with unpurified ab109517 on tissue sections from chicken hindbrain. The sections were blocked with 3% BSA for 1 hour at 4°C, before incubation with unpurified ab109517 (1/1000 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor 568 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.

    See Abreview

  • Anti-Islet 1 antibody [EP4182] (ab109517) at 1/50000 dilution (purified) + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 39 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/Immunofluorescence analysis of U-87 MG cells labelling Islet 1 with unpurified ab109517.

  • Anti-Islet 1 antibody [EP4182] (ab109517) at 1/50000 dilution (purified) + K562 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 39 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-Islet 1 antibody [EP4182] (ab109517) at 1/10000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 39 kDa
  • ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/1,500) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat whole cell lysate.

References for Anti-Islet 1 antibody [EP4182] (ab109517)

This product has been referenced in:
  • Michalak SM  et al. Ocular Motor Nerve Development in the Presence and Absence of Extraocular Muscle. Invest Ophthalmol Vis Sci 58:2388-2396 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28437527) »
  • Wang Y  et al. ISL1 and JMJD3 synergistically control cardiac differentiation of embryonic stem cells. Nucleic Acids Res 44:6741-55 (2016). IP ; Human . Read more (PubMed: 27105846) »

See all 6 Publications for this product

Product Wall

Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - nuclear (Min6)
Specification
Min6
Negative control
Negative control for Isl-1 antibody: known locus without enrichment Positive control for IP: Antibody for another factor that is not enriched at that locus
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1.1% Formaldehyed
Positive control
Positive control for Isl-1 antibody: known locus of enrichment Positive control for IP: Antibody for another factor also enriched at that locus
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Submitted Mar 24 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde
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Submitted Oct 16 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample
Chicken Tissue sections (hindbrain section)
Specification
hindbrain section
Permeabilization
Yes - Triton-X
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Oct 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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