Overview

  • Product nameAnti-Islet 2 antibody
    See all Islet 2 primary antibodies
  • Description
    Mouse monoclonal to Islet 2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 261-359 of Human Islet 2 (NP_665804)

  • Positive control
    • Islet 2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionTranscriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10160 antibody
    • Insulin gene enhancer protein ISL 2 antibody
    • Insulin gene enhancer protein ISL-2 antibody
    • Insulin gene enhancer protein ISL2 antibody
    • ISL 2 antibody
    • ISL 2 transcription factor antibody
    • ISL 2 transcription factor LIM/homeodomain antibody
    • ISL LIM homeobox 2 antibody
    • ISL2 antibody
    • ISL2 transcription factor antibody
    • ISL2 transcription factor LIM/homeodomain antibody
    • ISL2_HUMAN antibody
    • Islet-2 antibody
    • Islet2 antibody
    • SL2 transcription factor LIM/homeodomain (islet-2) antibody
    see all

Anti-Islet 2 antibody images

  • Anti-Islet 2 antibody (ab77212) at 5 µg/ml + Recombinant fragment of human Islet 2 at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 40 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Islet 2 antibody (ab77212) at 5 µg/ml

    Lane 1 : Islet 2 transfected lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 40 kDa
    Observed band size : 40 kDa

References for Anti-Islet 2 antibody (ab77212)

ab77212 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"