Overview

  • Product name
  • Description
    Rabbit polyclonal to ISM2
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    amino acids 138-209 (LREEEEARLL PRTHLQAELH QHGCWTVTEP AALTPGNATP PRTQEVTPLL LELQKLPELV HATLSTPNPD NQ) of the Human ISM2 protein Isoforms 1 and 3 (Q6H9L7).

  • Positive control
    • Human placenta tissue, Human Plasma lysate and Human cell line U-251 MG.

Properties

Applications

Our Abpromise guarantee covers the use of ab122547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • Tissue specificity
    Expressed at high levels in the placenta and at moderate levels in the pancreas, kidney, heart, liver, lung, brain and skeletal muscle.
  • Sequence similarities
    Belongs to the isthmin family.
    Contains 1 AMOP domain.
    Contains 1 TSP type-1 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gm264 antibody
    • ISM2 antibody
    • ISM2_HUMAN antibody
    • Isthmin-2 antibody
    • PSEC0137 antibody
    • TAIL1 antibody
    • Thrombospondin and AMOP containing isthmin like 1 antibody
    • Thrombospondin and AMOP domain-containing isthmin-like protein 1 antibody
    • Thrombospondin type-1 domain-containing protein 3 antibody
    • Thrombospondin, type I domain containing 3 antibody
    • Thrombospondin, type I, domain containing 3 antibody
    • THSD3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling ISM2 with ab122547. Staining shows strong cytoplasmic positivity in trophoblastic cells.
  • ab122547, at 4 µg/ml, staining ISM2 in Human cell line U-251 MG by Immunofluoroscence (Fixation/Permeabilization: PFA/Triton X-100).

References

ab122547 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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