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amino acids 138-209 (LREEEEARLL PRTHLQAELH QHGCWTVTEP AALTPGNATP PRTQEVTPLL LELQKLPELV HATLSTPNPD NQ) of the Human ISM2 protein Isoforms 1 and 3 (Q6H9L7).
Our Abpromise guarantee covers the use of ab122547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.|
ab122547, at 4 µg/ml, staining ISM2 in Human cell line U-251 MG by Immunofluoroscence (Fixation/Permeabilization: PFA/Triton X-100).
ab122547 has not yet been referenced specifically in any publications.