Anti-Isocitrate dehydrogenase antibody (ab81653)

Overview

  • Product nameAnti-Isocitrate dehydrogenase antibody
    See all Isocitrate dehydrogenase primary antibodies
  • Description
    Rabbit polyclonal to Isocitrate dehydrogenase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster
  • Immunogen

    Synthetic peptide from within residues: LGMMTSVLVC PDGKTVEAEA AHGTVTRHYR MYQKGQETST NPIASIFAWT, corresponding to internal sequence amino acids 288-337 of Human Isocitrate dehydrogenase (NP_005887)

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

Target

Anti-Isocitrate dehydrogenase antibody images

  • Rabbit Anti-IDH1 Antibody ab81653. Paraffin Embedded Tissue: Human Testis. Antibody Concentration: 5 ug/ml
  • Anti-Isocitrate dehydrogenase antibody (ab81653) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Sample Type : Human glioma cells. Primary Antibody Dilution : 1:200. Secondary Antibody : Anti-rabbit-GFP. Secondary Antibody Dilution : 1:5000. Color/Signal Descriptions : 1. Red: Nucleus 2. Green: IDH 3. Merge. Gene Name : IDH1.
  • ab81653 at 5 µg/ml staining Isocitrate dehydrogenase in Human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab81653 at 5 µg/ml staining Isocitrate dehydrogenase in Human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-Isocitrate dehydrogenase antibody (ab81653)

ab81653 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Prostate cancer cell line)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 20 µg
Specification Prostate cancer cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Submitted Jun 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"