Anti-Isocitrate dehydrogenase antibody (ab85532)

Overview

  • Product nameAnti-Isocitrate dehydrogenase antibody
    See all Isocitrate dehydrogenase primary antibodies
  • Description
    Rabbit polyclonal to Isocitrate dehydrogenase
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse
  • Immunogen

    Purified Isocitrate dehydrogenase from Pig heart.

  • Positive control
    • Heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab85532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
ELISA 1/400 - 1/1600.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Involvement in diseaseGlioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
  • Sequence similaritiesBelongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational
    modifications
    Acetylation at Lys-374 dramatically reduces catalytic activity.
  • Cellular localizationCytoplasm. Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytosolic NADP isocitrate dehydrogenase antibody
    • Cytosolic NADP-isocitrate dehydrogenase antibody
    • Epididymis luminal protein 216 antibody
    • Epididymis secretory protein Li 26 antibody
    • HEL-216 antibody
    • HEL-S-26 antibody
    • ICDH antibody
    • IDCD antibody
    • IDH antibody
    • IDH1 antibody
    • IDHC_HUMAN antibody
    • IDP antibody
    • IDPC antibody
    • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
    • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
    • NADP dependent isocitrate dehydrogenase cytosolic antibody
    • NADP dependent isocitrate dehydrogenase peroxisomal antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    • PICD antibody
    see all

Anti-Isocitrate dehydrogenase antibody images

  • ab85532 at 10µg/ml staining Isocitrate dehydrogenase in human heart tissue, by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue

References for Anti-Isocitrate dehydrogenase antibody (ab85532)

ab85532 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

As I understand it, if the recommended IHC working concentration for this antibody is 10ug/ml, and 100ul are needed for slide, then you would need 1ug of antibody per 100ul of diluent, that is, 1ug of antibody per...

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Thank you for contacting us. I would say that this sort of experiment is very-very tricky. We unfortunately do not have any protocol available at the moment for manually IHC staining quantification with antibodies. I would suggest ploughing through...

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Thank you for your enquiry. This antibody was raised against isocytrate dehydrogenase purified from porcine heart. According to our collaborator who developed the antibody, they have only tested it against wild type isocytrate dehydrogenase. It h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"