Anti-Isocitrate dehydrogenase antibody (ab94571)

Overview

  • Product nameAnti-Isocitrate dehydrogenase antibody
    See all Isocitrate dehydrogenase primary antibodies
  • Description
    Rabbit polyclonal to Isocitrate dehydrogenase
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Isocitrate dehydrogenase.

  • Positive control
    • This antibody gave a positive signal in HepG2 whole cell lysate as well as the following tissue lysates: Human kidney; Human testis. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab94571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Involvement in diseaseGlioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
  • Sequence similaritiesBelongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational
    modifications
    Acetylation at Lys-374 dramatically reduces catalytic activity.
  • Cellular localizationCytoplasm. Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytosolic NADP isocitrate dehydrogenase antibody
    • Cytosolic NADP-isocitrate dehydrogenase antibody
    • Epididymis luminal protein 216 antibody
    • Epididymis secretory protein Li 26 antibody
    • HEL-216 antibody
    • HEL-S-26 antibody
    • ICDH antibody
    • IDCD antibody
    • IDH antibody
    • IDH1 antibody
    • IDHC_HUMAN antibody
    • IDP antibody
    • IDPC antibody
    • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
    • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
    • NADP dependent isocitrate dehydrogenase cytosolic antibody
    • NADP dependent isocitrate dehydrogenase peroxisomal antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    • PICD antibody
    see all

Anti-Isocitrate dehydrogenase antibody images

  • All lanes : Anti-Isocitrate dehydrogenase antibody (ab94571) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 28 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ICC/IF image of ab94571 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94571, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 5µg/ml.
  • IHC image of Isocitrate dehydrogenase staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94571, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Isocitrate dehydrogenase antibody (ab94571)

ab94571 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"