Anti-Isoleucyl tRNA synthetase antibody (ab31533)

Overview

  • Product nameAnti-Isoleucyl tRNA synthetase antibody
    See all Isoleucyl tRNA synthetase primary antibodies
  • Description
    Rabbit polyclonal to Isoleucyl tRNA synthetase
  • SpecificityThis antibody reacts specifically with Isoleucyl tRNA synthetase protein.
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    S1074-F1264 denatured recombinant human IRS.

Properties

Applications

Our Abpromise guarantee covers the use of ab31533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 145 kDa.
IP Use at an assay dependent dilution.
ICC/IF 1/1000.

Target

  • RelevanceIsoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • FLJ20736 antibody
    • IARS antibody
    • IARS1 antibody
    • IleRS antibody
    • ILRS antibody
    • IRS antibody
    • Isoleucine tRNA ligase 1 cytoplasmic antibody
    • Isoleucine tRNA ligase antibody
    • Isoleucine tRNA synthetase antibody
    • Isoleucyl tRNA synthetase antibody
    • PRO0785 antibody
    see all

Anti-Isoleucyl tRNA synthetase antibody images

  • ICC/IF image of ab31533 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31533, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Isoleucyl tRNA synthetase antibody (ab31533) at 1/2000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 145 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

References for Anti-Isoleucyl tRNA synthetase antibody (ab31533)

ab31533 has not yet been referenced specifically in any publications.

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