Anti-Isoleucyl tRNA synthetase antibody (ab76971)

Overview

  • Product nameAnti-Isoleucyl tRNA synthetase antibody
    See all Isoleucyl tRNA synthetase primary antibodies
  • Description
    Mouse monoclonal to Isoleucyl tRNA synthetase
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment: QYINLQLLNA KPQECLMGTV GTLLLENPLG QNGLTHQGLL YEAAKVFGLR SRKLKLFLNE TQTQEITEDI PVKTLNMKTV YVSVLPTTAD F, corresponding to amino acids 1172-1262 of human Isoleucyl tRNA synthetase (NP_038203) with a 26 kDa tag.

  • Positive control
    • Isoleucyl tRNA synthetase transfected 293T cell lysate; immunogen

Properties

Applications

Our Abpromise guarantee covers the use of ab76971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 145 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceIsoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • FLJ20736 antibody
    • IARS antibody
    • IARS1 antibody
    • IleRS antibody
    • ILRS antibody
    • IRS antibody
    • Isoleucine tRNA ligase 1 cytoplasmic antibody
    • Isoleucine tRNA ligase antibody
    • Isoleucine tRNA synthetase antibody
    • Isoleucyl tRNA synthetase antibody
    • PRO0785 antibody
    see all

Anti-Isoleucyl tRNA synthetase antibody images

  • All lanes : Anti-Isoleucyl tRNA synthetase antibody (ab76971) at 5 µg/ml

    Lane 1 : Isoleucyl tRNA synthetase transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 145 kDa
    Observed band size : 121 kDa (why is the actual band size different from the predicted?)
  • Anti-Isoleucyl tRNA synthetase antibody (ab76971) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 145 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References for Anti-Isoleucyl tRNA synthetase antibody (ab76971)

ab76971 has not yet been referenced specifically in any publications.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"