Recombinant Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)] (ab151557)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10266(B)] to Isoleucyl tRNA synthetase
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)]
See all Isoleucyl tRNA synthetase primary antibodies -
Description
Rabbit monoclonal [EPR10266(B)] to Isoleucyl tRNA synthetase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Isoleucyl tRNA synthetase aa 1-100 (N terminal). The exact sequence is proprietary.
-
Positive control
- HeLa, Ramos, Jurkat and HepG2 cell lysates; HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10266(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
|
|
ICC/IF |
1/100 - 1/250.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 145 kDa. |
ICC/IF
1/100 - 1/250. |
Target
-
Relevance
Isoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3376 Human
- Omim: 600709 Human
- SwissProt: P41252 Human
-
Alternative names
- FLJ20736 antibody
- IARS antibody
- IARS1 antibody
see all
Images
-
All lanes : Anti-Isoleucyl tRNA synthetase antibody [EPR10266(B)] (ab151557)
Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 145 kDa -
Immunofluorescent analysis of HeLa cells labeling Isoleucyl tRNA synthetase with ab151557 at 1/1000 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab151557 has not yet been referenced specifically in any publications.