Overview

  • Product name
    Anti-Isopeptide antibody [81D1C2]
  • Description
    Mouse monoclonal [81D1C2] to Isopeptide
  • Specificity
    This antibody reacts with the isopeptide : epsilon-(gamma-glutamyl) lysine. There is no cross reaction with either lysine or glutamine. They are specific to the epsilon-(gamma-glutamyl) lysine bond formed by transglutaminase activity.
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Synthetic isopeptide.

  • General notes
    The ability of this antibody to fix complement is unknown. The isopeptide may be purchased from Bachem Catalog number G-1970.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    81D1C2
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/500.

Target

  • Alternative names
    • Isopeptide bond antibody

References

This product has been referenced in:
  • Liu C  et al. Tissue transglutaminase contributes to the pathogenesis of preeclampsia and stabilizes placental angiotensin receptor type 1 by ubiquitination-preventing isopeptide modification. Hypertension 63:353-61 (2014). WB . Read more (PubMed: 24191290) »
  • Llano E  et al. STAG3 is a strong candidate gene for male infertility. Hum Mol Genet 23:3421-31 (2014). Read more (PubMed: 24608227) »

See all 8 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Human umbilical artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH=8
Permeabilization
No
Specification
Human umbilical artery
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Prof. Jin-Jia Hu

Verified customer

Submitted May 11 2015

Antigen: BSA; ab422 is a monoclonal and does not need to be pre-absorbed.

As far as we are aware, ab422 has not yet been tested in in flow cytometry. We do not stock a labelled version of this antibody. ab8828 also has not yet been tested in flow cytometry. If you do test these antibodies in FC, please let us know how you g...

Read More

The MAb to isopeptide shows good reactivity on cross linked protein containing isopeptide N-epsilon(gamma-glutamyl)Lys but has weak reactivity on free isopeptide. Therefore commercialy available free isopeptide from Sigma Chemical is not a good positi...

Read More

The difference between these two antibodies is the isotype: 153-81D4 is an IgM and 153-81D1C2 is an IgG1. They can both be used for both applications, so the choice is yours.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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