Overview

  • Product nameAnti-Isopeptide antibody [81D1C2]
  • Description
    Mouse monoclonal [81D1C2] to Isopeptide
  • SpecificityThis antibody reacts with the isopeptide : epsilon-(gamma-glutamyl) lysine. There is no cross reaction with either lysine or glutamine. They are specific to the epsilon-(gamma-glutamyl) lysine bond formed by transglutaminase activity.
  • Tested applicationsSuitable for: WBmore details
  • Immunogen

    Synthetic isopeptide.

  • General notesThe ability of this antibody to fix complement is unknown. The isopeptide may be purchased from Bachem Catalog number G-1970.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number81D1C2
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/500.

Target

  • Alternative names
    • Isopeptide bond antibody

References for Anti-Isopeptide antibody [81D1C2] (ab422)

This product has been referenced in:
  • Liu C  et al. Tissue transglutaminase contributes to the pathogenesis of preeclampsia and stabilizes placental angiotensin receptor type 1 by ubiquitination-preventing isopeptide modification. Hypertension 63:353-61 (2014). WB . Read more (PubMed: 24191290) »
  • L'Heureux DZ  et al. The interaction of angiocidin with tissue transglutaminase. Exp Mol Pathol 88:15-25 (2010). Read more (PubMed: 19931242) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Human umbilical artery)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH=8
Permeabilization No
Specification Human umbilical artery
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Prof. Jin-Jia Hu

Verified customer

Submitted May 11 2015

Antigen: BSA; ab422 is a monoclonal and does not need to be pre-absorbed.

As far as we are aware, ab422 has not yet been tested in in flow cytometry. We do not stock a labelled version of this antibody. ab8828 also has not yet been tested in flow cytometry. If you do test these antibodies in FC, please let us know how you g...

Read More

The MAb to isopeptide shows good reactivity on cross linked protein containing isopeptide N-epsilon(gamma-glutamyl)Lys but has weak reactivity on free isopeptide. Therefore commercialy available free isopeptide from Sigma Chemical is not a good positi...

Read More

The difference between these two antibodies is the isotype: 153-81D4 is an IgM and 153-81D1C2 is an IgG1. They can both be used for both applications, so the choice is yours.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"