Isoprenaline hydrochloride (Isoproterenol) (ab120710)

Overview

  • Product nameIsoprenaline hydrochloride (Isoproterenol)
  • Description
    Non-selective β adrenoceptor agonist
  • Alternative names
    • Isoproterenol
  • Biological descriptionNon-selective β adrenoceptor agonist. Structurally similar to adrenaline. Stimulates MAPK activation through cAMP dependent kinases (EC50 = 1-3 μM). Systemically active upon subcutaneous administration.

    Also available in simple stock solutions (ab146724) - add 1 ml of water to get an exact, ready-to-use concentration.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120710 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Isoprenaline hydrochloride (Isoproterenol) images

    • ab62153 staining HSL pSer855 in NIH-3T3 cells treated with isoprenaline hydrochloride (ab120710), by ICC/IF. Increase of HSL pSer855 expression correlates with increased concentration of isoprenaline hydrochloride, as described in literature.
      The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120710 (isoprenaline hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab62153 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Isoprenaline hydrochloride (Isoproterenol) (ab120710)

    This product has been referenced in:
    • Nagoor Meeran MF  et al. Preventive effects of N-acetyl cysteine on lipids, lipoproteins and myocardial infarct size in isoproterenol induced myocardial infarcted rats: an in vivo and in vitro study. Eur J Pharmacol 677:116-22 (2012). Read more (PubMed: 22178923) »
    • Brouri F  et al. Blockade of beta 1- and desensitization of beta 2-adrenoceptors reduce isoprenaline-induced cardiac fibrosis. Eur J Pharmacol 485:227-34 (2004). Read more (PubMed: 14757145) »
    • Schmitt JM & Stork PJ beta 2-adrenergic receptor activates extracellular signal-regulated kinases (ERKs) via the small G protein rap1 and the serine/threonine kinase B-Raf. J Biol Chem 275:25342-50 (2000). Read more (PubMed: 10840035) »

    See 0 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab120710.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"