Overview

  • Product nameAnti-IST1 antibody [22]
    See all IST1 primary antibodies
  • Description
    Mouse monoclonal [22] to IST1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein expressed in E. Coli

  • Positive control
    • H520 whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Proclin
    Constituents: Citrate-Tris HCl, pH 7.0
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number22
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 40 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionProposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
  • Sequence similaritiesBelongs to the IST1 family.
  • Cellular localizationCytoplasmic vesicle. Localizes to the midbody of dividing cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • hIST1 antibody
    • Increased sodium tolerance 1 antibody
    • Increased sodium tolerance 1 homolog antibody
    • IST1 homolog antibody
    • IST1_HUMAN antibody
    • KIAA0174 antibody
    • MAPK activating protein PM28 antibody
    • MGC117220 antibody
    • MGC128433 antibody
    • Putative MAPK-activating protein PM28 antibody
    see all

Anti-IST1 antibody [22] images

  • Anti-IST1 antibody [22] (ab87456) at 1/1000 dilution + H520 whole cell lysate

    Predicted band size : 40 kDa

References for Anti-IST1 antibody [22] (ab87456)

This product has been referenced in:
  • Lee S  et al. MITD1 is recruited to midbodies by ESCRT-III and participates in cytokinesis. Mol Biol Cell 23:4347-61 (2012). ICC/IF . Read more (PubMed: 23015756) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"