The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 40 kDa.
Use at an assay dependent dilution.
FunctionProposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
Sequence similaritiesBelongs to the IST1 family.
Cellular localizationCytoplasmic vesicle. Localizes to the midbody of dividing cells.