Overview

  • Product name
    Anti-ISW1-N antibody
  • Description
    Mouse polyclonal to ISW1-N
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to Saccharomyces cerevisiae ISW1-N aa 1-30. (NP_009804.1)
    Sequence:

    MAYMLAIANFHFFKFYTRMRKKHENNSCNE


    Database link: P38144

  • General notes

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein.
    A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
    Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab43434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ICC/IF 1/1000.
IP 1/1000.

Target

  • Relevance
    Catalytic component of ISW1-type complexes, which act by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. They are involved in coordinating transcriptional repression, activation and elongation phases. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome. The ISW1B complex acts within coding regions to control the amount of RNA polymerase II released into productive elongation and to coordinate elongation with termination and pre-mRNA processing
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • ISW1 antibody
      • ISWI chromatin-remodeling complex ATPase ISW1 antibody
      • YBR1633 antibody
      • YBR245C antibody

    References

    ab43434 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (whole cell lysate)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    40 µg
    Specification
    whole cell lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 04 2017

    Abreviews
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (S288C)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    3.107 cells
    Specification
    S288C
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mrs. Anna Babour

    Verified customer

    Submitted Sep 04 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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