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- Proteins and Peptides
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Buying a fast track?
We are able to bring you this product at a low price as it is not yet fully characterized. Our Scientific Support team are available to assist you, but we cannot offer refunds or replacements on this product.
What is a fast track? »Overview
Product name
Anti-ISWI (acetyl K753) antibody
Description
Rabbit polyclonal to ISWI (acetyl K753)
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Specificity
The specificity of the antibody can be summarized as follows: 1. On Western blots probing recombinant ISWI which had been (or not) acetylated at K753 by GCN5, the antibody recognizes the acetylated ISWI with very high selectivity. No reaction with unacetylated ISWI has been observed. 2. Unexpectedly, there is a significant cross-reactivity with GCN5 under acetylation conditions (we assume autoacetylated GCN5). As shown in the figure, this cross-reactivity is low, since conditions can be found easily where the GCN5 band on Western blots is not observed. 3. In whole cell extracts the antibody cross-reacts with a number of small molecular weight bands. A faint signal at the position of ISWI is detected as well, which we think corresponds to ISWI since it is not present if ISWI is ablated using RNAi. The weakness of the signal may simply indicate that there is very little epitope present in these cells. In summary, we are confident that the antibody can be used on purified ISWI (e.g. ISWI immunoprecipitated from a cruder source).
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 700 - 800 of Fruit fly (Drosophila melanogaster) ISWI, acetylated at K753.
(Peptide available as ab160 64.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> ISWI/NURF/CHRAC
Applications
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Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).Fast track terms of use
ELISA: Use at an assay dependent dilution. This antibody gave a positive result in ELISA against the immunizing peptide (ab16064). It gave a negative result in ELISA against the non-modified equivalent peptide (ab96245). This indicates that it is specific for the modified peptide.
Target
Relevance
ISWI is a component of the nucleosome remodeling factor complex (NURF), a protein complex that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. The hydrolysis of ATP during the remodeling of chromatin is likely to be mediated by ISWI, releasing inorganic phosphate. It is also a component of the ATP-utilizing chromatin assembly and remodeling factor (ACF) and of the chromatin accessibility complex (CHRAC). This subunit may serve as the energy-transducing component of chromatin-remodeling machines.
Cellular localization
Nuclear
Alternative names
- CHRAC 140 kDa subunit antibody
- Chromatin remodelling complex ATPase chain Iswi antibody
- dISWI antibody
see all
Database links
- Entrez Gene: 36390 Fruit fly (Drosophila melanogaster)
- SwissProt: Q24368 Fruit fly (Drosophila melanogaster)
Anti-ISWI (acetyl K753) antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - ISWI (acetyl K753) antibody (ab10748)
Lanes 1 - 2 : Anti-ISWI (acetyl K753) antibody (ab10748) at 1/2500 dilution
Lanes 3 - 4 : Anti-ISWI (acetyl K753) antibody (ab10748) at 1/500 dilution
Lane 1 : As above
Lane 2 : Recombinant ISWI (200 ng) was acetylated using 125ng of hGCN5 and a final concentration of 6 µM of 3H-AcCoA.
Lane 3 : Recombinant ISWI (200 ng) was acetylated using 125ng of hGCN5 and a final concentration of 6 µM of 3H-AcCoA.
Lane 4 : As above
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
In lanes 3 and 4, 1/5th of the reaction was loaded.
Peter Becker
Western blot - ISWI (acetyl K753) antibody (ab10748)
All lanes : Anti-ISWI (acetyl K753) antibody (ab10748) at 1/500 dilution
Lane 1 : Drosophila melanogaster SL-2 cells treated for 8 days with ISWI dsRNAs
Lane 2 : Drosophila melanogaster SL-2 cells treated for 8 days with control dsRNAs
Lysates/proteins at 30 µg per lane.
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa,60 kDa,65 kDa. We are unsure as to the identity of these extra bands.
Peter Becker
References for Anti-ISWI (acetyl K753) antibody (ab10748)
This product has been referenced in:
- Li M et al. Modulation of chromatin boundary activities by nucleosome-remodeling activities in Drosophila melanogaster. Mol Cell Biol 30:1067-76 (2010). ChIP; Fruit fly (Drosophila melanogaster).Read more (PubMed: 19995906) »
See 1 publication for this product
Publishing research using ab10748? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"