Overview

  • Product name
  • Description
    Rabbit polyclonal to ISY1
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 174-285 of Human ISY1.

  • Positive control
    • Human stomach slides, RT-4, U-251 MG, human liver, and human tonsil lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 60% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function
    May play a role in pre-mRNA splicing.
  • Sequence similarities
    Belongs to the ISY1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FSAP33 antibody
    • ISY1 antibody
    • ISY1 splicing factor homolog (S. cerevisiae) antibody
    • ISY1_HUMAN antibody
    • KIAA1160 antibody
    • Pre-mRNA-splicing factor ISY1 homolog antibody
    see all

Images

  • ab121523, at 1-4µg/mL, staining human cell line U-2 OS by immunofluorescence.
  • ab121523, at 1/600 dilution, staining human stomach by immunohistochemistry on paraffin embedded tissues (IHC-P).
  • Immunohistochemical staining of Human stomach shows nuclear positivity in glandular cells using ab121523

References

ab121523 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121523.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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