• Product nameAnti-ITCH/AIP4 antibody [EPR4937]
    See all ITCH/AIP4 primary antibodies
  • Description
    Rabbit monoclonal [EPR4937] to ITCH/AIP4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • K562, HL60, HeLa, and MCF-7 cell lysates; Human breast tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab109018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 103 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionActs as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteosomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Critical regulator of T helper (TH2) cytokine development through its ability to induce JUNB ubiquitination and degradation (By similarity). Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It is involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteosomal degradation. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteosomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteosomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination.
    • Tissue specificityWidely expressed.
    • PathwayProtein modification; protein ubiquitination.
    • Involvement in diseaseDefects in ITCH are the cause of syndromic multisystem autoimmune disease (SMAD) [MIM:613385]. SMAD is characterized by organomegaly, failure to thrive, developmental delay, dysmorphic features and autoimmune inflammatory cell infiltration of the lungs, liver and gut.
    • Sequence similaritiesContains 1 C2 domain.
      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
      Contains 4 WW domains.
    • Post-translational
      On T-cell activation, phosphorylation by the JNK cascade on serine and threonine residues surrounding the PRR domain accelerates the ubiquitination and degradation of JUN and JUNB. The increased ITCH catalytic activity due to phosphorylation by JNK1 may occur due to a conformational change disrupting the interaction between the PRR/WW motifs domain and the HECT domain and, thus exposing the HECT domain (By similarity). Phosphorylation by FYN reduces interaction with JUNB and negatively controls JUN ubiquitination and degradation.
      Ubiquitinated; autopolyubiquitination with 'Lys-63' linkages which does not lead to protein degradation.
    • Cellular localizationCell membrane. Cytoplasm. Nucleus. Associates with endocytic vesicles. May be recruited to exosomes by NDFIP1.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADMFD antibody
      • AIF4 antibody
      • AIP4 antibody
      • Atrophin 1 interacting protein 4 antibody
      • Atrophin-1-interacting protein 4 antibody
      • dJ468O1.1 antibody
      • dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)) antibody
      • dJ468O1.1 atrophin 1 interacting protein 4 AIP4 antibody
      • E3 ubiquitin protein ligase Itchy homolog antibody
      • E3 ubiquitin-protein ligase Itchy homolog antibody
      • EC 6.3.2 antibody
      • Itch antibody
      • ITCH_HUMAN antibody
      • Itchy E3 ubiquitin protein ligase antibody
      • Itchy E3 ubiquitin protein ligase homolog antibody
      • Itchy E3 ubiquitin protein ligase homolog mouse antibody
      • Itchy E3 ubiquitin protein ligase, mouse, homolog of antibody
      • Itchy homolog E3 ubiquitin protein ligase antibody
      • Itchy mouse homolog E3 ubiquitin protein ligase antibody
      • NAPP1 antibody
      • NFE2 associated polypeptide 1 antibody
      • NFE2-associated polypeptide 1 antibody
      • Ubiquitin protein ligase ITCH antibody
      see all

    Anti-ITCH/AIP4 antibody [EPR4937] images

    • All lanes : Anti-ITCH/AIP4 antibody [EPR4937] (ab109018) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HL60 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 103 kDa
    • Immunohistochemical analysis of ITCH/AIP4 expression in paraffin-embedded Human breast tissue using ab109018 at 1/500 dilution.

    References for Anti-ITCH/AIP4 antibody [EPR4937] (ab109018)

    This product has been referenced in:
    • Holleman J & Marchese A The ubiquitin ligase deltex-3l regulates endosomal sorting of the G protein-coupled receptor CXCR4. Mol Biol Cell 25:1892-904 (2014). WB . Read more (PubMed: 24790097) »

    See 1 Publication for this product

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    ab74037 to be tested in IHC-P: discount code ****
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