Anti-ITGA7 antibody [3C12] (ab195959)
Key features and details
- Mouse monoclonal [3C12] to ITGA7
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-ITGA7 antibody [3C12]
See all ITGA7 primary antibodies -
Description
Mouse monoclonal [3C12] to ITGA7 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Tissue, cells or virus corresponding to Mouse ITGA7. ab195959 was raised against Mouse myoblasts.
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Positive control
- Human brain, cortex tissue; C2C12 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3C12 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 10 - 20 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt
Use a concentration of 10 - 20 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity). Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells. -
Tissue specificity
Isoforms containing segment A are predominantly expressed in skeletal muscle. Isoforms containing segment B are abundantly expressed in skeletal muscle, moderately in cardiac muscle, small intestine, colon, ovary and prostate and weakly in lung and testes. Isoforms containing segment X2D are expressed at low levels in fetal and adult skeletal muscle and in cardiac muscle, but are not detected in myoblasts and myotubes. In muscle fibers isoforms containing segment A and B are expressed at myotendinous and neuromuscular junctions; isoforms containing segment C are expressed at neuromuscular junctions and at extrasynaptic sites. Isoforms containing segments X1 or X2 or, at low levels, X1X2 are expressed in fetal and adult skeletal muscle (myoblasts and myotubes) and cardiac muscle. -
Involvement in disease
Defects in ITGA7 are the cause of muscular dystrophy congenital due to integrin alpha-7 deficiency (MDCI) [MIM:613204]. A form of congenital muscular dystrophy. Patients present at birth, or within the first few months of life, with hypotonia, muscle weakness and often with joint contractures. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Developmental stage
In renewing intestinal epithelium, expression of isoforms containing segment B correlates with the onset of enterocytic differentiation. -
Post-translational
modificationsADP-ribosylated on at least two sites of the extracellular domain in skeletal myotubes.
A 70 kDa form is created by proteolytic cleavage. Cleavage is elevated during myogenic differentiation and the cleaved form enhances cell adhesion and spreading on laminin. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3679 Human
- Entrez Gene: 16404 Mouse
- Omim: 600536 Human
- SwissProt: Q13683 Human
- SwissProt: Q61738 Mouse
- Unigene: 524484 Human
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Alternative names
- alpha 7 antibody
- FLJ25220 antibody
- Integrin alpha 7 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab195959 has been referenced in 2 publications.
- Kobayashi N et al. Integrin a7 and Extracellular Matrix Laminin 211 Interaction Promotes Proliferation of Acute Myeloid Leukemia Cells and Is Associated with Granulocytic Sarcoma. Cancers (Basel) 12:N/A (2020). PubMed: 32033262
- Engl T et al. Acquired resistance to temsirolimus is associated with integrin a7 driven chemotactic activity of renal cell carcinoma in vitro. Oncotarget 9:18747-18759 (2018). PubMed: 29721158