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Synthetic peptide derived from human ITGA7
Our Abpromise guarantee covers the use of ab75224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 129 kDa. for the full length protein.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/100. (see Abreview)|
ab75224 staining ITGA7 (green) in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 20 minutes at 22°C; antigen retrieval was by heat mediation in a tris buffer. Samples were incubated with primary antibody (1/100) for 12 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. 10x objective.