Overview

  • Product nameAnti-ITGA7 antibody
    See all ITGA7 primary antibodies
  • Description
    Rabbit polyclonal to ITGA7
  • Specificityab75224 detects endogenous levels of a fragment of activated ITGA7 resulting from cleavage adjacent to Glu959.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human ITGA7

  • Positive control
    • COS-7 cells treated with etoposide (25 µM, 1 hour),

Properties

Applications

Our Abpromise guarantee covers the use of ab75224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 129 kDa. for the full length protein.
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/100. (see Abreview)

Target

  • FunctionIntegrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity). Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells.
  • Tissue specificityIsoforms containing segment A are predominantly expressed in skeletal muscle. Isoforms containing segment B are abundantly expressed in skeletal muscle, moderately in cardiac muscle, small intestine, colon, ovary and prostate and weakly in lung and testes. Isoforms containing segment X2D are expressed at low levels in fetal and adult skeletal muscle and in cardiac muscle, but are not detected in myoblasts and myotubes. In muscle fibers isoforms containing segment A and B are expressed at myotendinous and neuromuscular junctions; isoforms containing segment C are expressed at neuromuscular junctions and at extrasynaptic sites. Isoforms containing segments X1 or X2 or, at low levels, X1X2 are expressed in fetal and adult skeletal muscle (myoblasts and myotubes) and cardiac muscle.
  • Involvement in diseaseDefects in ITGA7 are the cause of muscular dystrophy congenital due to integrin alpha-7 deficiency (MDCI) [MIM:613204]. A form of congenital muscular dystrophy. Patients present at birth, or within the first few months of life, with hypotonia, muscle weakness and often with joint contractures.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Developmental stageIn renewing intestinal epithelium, expression of isoforms containing segment B correlates with the onset of enterocytic differentiation.
  • Post-translational
    modifications
    ADP-ribosylated on at least two sites of the extracellular domain in skeletal myotubes.
    A 70 kDa form is created by proteolytic cleavage. Cleavage is elevated during myogenic differentiation and the cleaved form enhances cell adhesion and spreading on laminin.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha 7 antibody
    • FLJ25220 antibody
    • Integrin alpha 7 antibody
    • Integrin alpha 7 chain antibody
    • Integrin alpha 7 heavy chain antibody
    • Integrin alpha 7 light chain antibody
    • Integrin alpha-7 70 kDa form antibody
    • ITA7_HUMAN antibody
    • ITGA 7 antibody
    • Itga7 antibody
    see all

Anti-ITGA7 antibody images

  • All lanes : Anti-ITGA7 antibody (ab75224) at 1/500 dilution

    Lane 1 : COS-7 cells, treated with etoposide (25 µM, 1 hour)
    Lane 2 : COS-7 cells, treated with etoposide (25 µM, 1 hour) with Cl-peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 129 kDa
    Additional bands at : 26 kDa (possible cleavage fragment).
  • ab75224 staining ITGA7 (green) in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 20 minutes at 22°C; antigen retrieval was by heat mediation in a tris buffer. Samples were incubated with primary antibody (1/100) for 12 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. 10x objective.

    See Abreview

  • ab75224 staining ITGA7 in Human skeletal muscle tissue by Immunohistochemistry (Frozen sections). The sections were permeabilized in Triton X-100 prior to blocking with 10% Goat serum for 1 hour at 25°C. The primary antibody was diluted 1/100 in 1xPBS/10% Goat Serum/0.1% Triton and incubated with the sample for 12 hours at 4°C. An Alexa Fluor 568®-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/250.

    See Abreview

References for Anti-ITGA7 antibody (ab75224)

This product has been referenced in:
  • Simon L  et al. Chronic binge alcohol consumption alters myogenic gene expression and reduces in vitro myogenic differentiation potential of myoblasts from rhesus macaques. Am J Physiol Regul Integr Comp Physiol 306:R837-44 (2014). Read more (PubMed: 24671243) »
  • Heller KN  et al. AAV-mediated overexpression of human a7 integrin leads to histological and functional improvement in dystrophic mice. Mol Ther 21:520-5 (2013). Human . Read more (PubMed: 23319059) »

See all 2 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris
Sample Human Tissue sections (Skeletal Muscle)
Specification Skeletal Muscle
Permeabilization No
Fixative Methacarn
Username

Abcam user community

Verified customer

Submitted Sep 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Skeletal Muscle)
Loading amount 20 µg
Specification Skeletal Muscle
Gel Running Conditions Reduced Denaturing (12% Bis-Tris gel)
Blocking step Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 03 2013

Thank you for contacting us.
I am sorry to hear that the antibody does not give you the 129 kDa band.

According to the Protein Database entry for human Integrin alpha 7 (http://www.uniprot.org/uniprot/Q13683), the protein is being cleaved ...

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Thank you for your response.

Your protocol is sound however there are a few things that you may want to try in order to get this product to perform satisfactorily in western blot.
I would consider using a different lysis buffer for your s...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skeletal muscle)
Specification Skeletal muscle
Fixative none
Permeabilization Yes - triton x-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Ms. Kristin Heller

Verified customer

Submitted Mar 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"