The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay play a role in the recruitment of beta-1 integrins to the focal contacts during integrin-dependent cell adhesion. Isoform 2 does not bind the integrin cytoplasmic domain-associated protein-1.
Tissue specificityExpressed in intestine, colon, testis, ovary, thymus, spleen and prostate.
Sequence similaritiesContains 1 PID domain.
Post-translational modificationsIsoform 1 appears to be phosphorylated. The degree of phosphorylation is regulated by integrin-dependent cell-matrix interaction.
ab115228, at 10 µg/ml, staining ITGB1BP1 in Human spleen tissue (formalin-fixed, paraffin-embedded) by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-ITGB1BP1 antibody (ab115228)
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