The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role in the recruitment of beta-1 integrins to the focal contacts during integrin-dependent cell adhesion. Isoform 2 does not bind the integrin cytoplasmic domain-associated protein-1.
Tissue specificityExpressed in intestine, colon, testis, ovary, thymus, spleen and prostate.
Sequence similaritiesContains 1 PID domain.
Post-translational modificationsIsoform 1 appears to be phosphorylated. The degree of phosphorylation is regulated by integrin-dependent cell-matrix interaction.