Overview

  • Product name
  • Description
    Rabbit polyclonal to ITGB1BP2
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: MSLLCRNKGC GQHFDPNTNL PDSCCHHPGV PIFHDALKGW SCCRKRTVDF, corresponding to N terminal amino acids 1-50 of Human ITGB1BP2

  • Positive control
    • Fetal heart cell lysate. Human muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab62300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Images

  • ITGB1BP2 antibody (ab62300) at 2.5µg/ml (in 5% skim milk / PBS buffer) + Fetal heart cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling ITGB1BP2 with ab62300 at 4-8µg/ml. Arrows indicate positively stained skeletal muscle cells. Magnification: 400X.

References

ab62300 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab62300.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up