The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
FunctionMay act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.
Sequence similaritiesBelongs to the ITIH family. Contains 1 VIT domain. Contains 1 VWFA domain.
Post-translational modificationsHeavy chains are linked to bikunin via chondroitin 4-sulfate esterified to the alpha-carboxyl of the C-terminal aspartate after propeptide cleavage. The S-linked glycan is composed of two 6-carbon sugars, possibly Glc or Gal.
Inter alpha (globulin) inhibitor H1 polypeptide antibody
Inter alpha trypsin inhibitor heavy chain 1 antibody
Inter alpha trypsin inhibitor heavy chain H1 antibody
Inter-alpha-inhibitor heavy chain 1 antibody
Inter-alpha-trypsin inhibitor complex component III antibody
Inter-alpha-trypsin inhibitor heavy chain H1 antibody
ITI heavy chain H1 antibody
Serum-derived hyaluronan-associated protein antibody
Anti-ITIH1 antibody images
Western blot - ITIH1 antibody (ab70048)
All lanes : Anti-ITIH1 antibody (ab70048) at 1/500 dilution
Lane 1 : ITIH1 transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 102 kDa Observed band size : 102 kDa
References for Anti-ITIH1 antibody (ab70048)
This product has been referenced in:
Zhang S et al. Constitutive expression of pentraxin 3 (PTX3) protein by human amniotic membrane cells leads to formation of the heavy chain (HC)-hyaluronan (HA)-PTX3 complex. J Biol Chem289:13531-42 (2014).
Read more (PubMed: 24652286) »
He H et al. Immobilized heavy chain-hyaluronic acid polarizes lipopolysaccharide-activated macrophages toward M2 phenotype. J Biol Chem288:25792-803 (2013).
Read more (PubMed: 23878196) »