Overview

  • Product nameAnti-ITIH3 antibody
    See all ITIH3 primary antibodies
  • Description
    Rabbit polyclonal to ITIH3
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment, corresponding to amino acids 119-353 of Human ITIH3 (NP_002208).

  • Positive control
    • A431 whole cell lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab97758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 100 kDa.

Target

  • RelevanceThe inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. ITIH3 may act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • AW108094 antibody
    • H3P antibody
    • Inter alpha (globulin) inhibitor, H3 polypeptide antibody
    • Inter alpha inhibitor heavy chain 3 antibody
    • Inter alpha trypsin inhibitor heavy chain H3 antibody
    • Intin3 antibody
    • ITI HC3 antibody
    • ITI heavy chain H3 antibody
    • PAIHC3 antibody
    • Pre alpha-inhibitor, heavy chain 3 antibody
    • Serum derived hyaluronan-associated protein antibody
    • SHAP antibody
    see all

Anti-ITIH3 antibody images

  • ICC/IF image of ab97758 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97758 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-ITIH3 antibody (ab97758) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 100 kDa
    7.5% SDS-PAGE.

References for Anti-ITIH3 antibody (ab97758)

This product has been referenced in:
  • Coulson-Thomas VJ  et al. Umbilical Cord Mesenchymal Stem Cells Suppress Host Rejection: THE ROLE OF THE GLYCOCALYX. J Biol Chem 289:23465-81 (2014). ICC/IF ; Human . Read more (PubMed: 24986866) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"