The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay be involved in acute phase reactions.
Tissue specificityLiver specific.
Sequence similaritiesBelongs to the ITIH family. Contains 1 VIT domain. Contains 1 VWFA domain.
Post-translational modificationsCleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment. Appears to be both N- and O-glycosylated. The O-linked polysaccharides on Ser-696, Thr-701 and Ser-702 are probably the mucin type linked to GalNAc.