Recombinant Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11233(2)] to ITIH4
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ITIH4 antibody [EPR11233(2)]
See all ITIH4 primary antibodies -
Description
Rabbit monoclonal [EPR11233(2)] to ITIH4 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human ITIH4 aa 1-150. The exact sequence is proprietary.
Database link: Q14624 -
Positive control
- Human and mouse plasma, Mouse and rat serum, Human liver and placenta tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11233(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
1/60.
|
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
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Notes |
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IP
1/60. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa). |
Target
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Function
May be involved in acute phase reactions. -
Tissue specificity
Liver specific. -
Sequence similarities
Belongs to the ITIH family.
Contains 1 VIT domain.
Contains 1 VWFA domain. -
Post-translational
modificationsCleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.
Appears to be both N- and O-glycosylated.
The O-linked polysaccharides on Ser-696, Thr-701 and Ser-702 are probably the mucin type linked to GalNAc. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3700 Human
- Entrez Gene: 16427 Mouse
- Entrez Gene: 54404 Rat
- NCBI: NP_061216.2 Mouse
- Omim: 600564 Human
- SwissProt: Q14624 Human
- SwissProt: Q9DBK8 Mouse
- Unigene: 709406 Human
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Alternative names
- 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 antibody
- gp120 antibody
- H4P antibody
see all
Images
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All lanes : Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse plasma
Lane 3 : Rat serum
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000 µg
Predicted band size: 103 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5%NFDM /TBST
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Western blot analysis of ITIH4 in immunoprecipitation pellets from Human plasma lysate. ab180139 used at 1/60 dilution for immunoprecipitation. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution + Human plasma at 10 µg
Secondary
Anti-Rabbit IgG (HRP) at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5%NFDM /TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab180139 has been referenced in 4 publications.
- Yin R et al. Proteomic landscape subtype and clinical prognosis of patients with the cognitive impairment by Japanese encephalitis infection. J Neuroinflammation 19:77 (2022). PubMed: 35379280
- Pihl R et al. ITIH4 acts as a protease inhibitor by a novel inhibitory mechanism. Sci Adv 7:N/A (2021). PubMed: 33523981
- Jing D et al. Effects of Helicobacter pylori infection and interleukin 6 on the expression of ITIH4 in human gastric cancer cells. Transl Cancer Res 9:4656-4665 (2020). PubMed: 35117829
- Kumagai T et al. Serum IgM Glycosylation Associated with Tuberculosis Infection in Mice. mSphere 4:N/A (2019). PubMed: 30918063