The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500.
1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
May be involved in acute phase reactions.
Belongs to the ITIH family. Contains 1 VIT domain. Contains 1 VWFA domain.
Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment. Appears to be both N- and O-glycosylated. The O-linked polysaccharides on Ser-696, Thr-701 and Ser-702 are probably the mucin type linked to GalNAc.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.
Western blot analysis of ITIH4 in immunoprecipitation pellets from Human plasma lysate. ab180139 used at 1/60 dilution for immunoprecipitation. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration: 5% NFDM/TBST.