ab107846 stained JEG3 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab107846 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Western blot - ITIH5 antibody (ab107846)
Anti-ITIH5 antibody (ab107846) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg
Secondary HRP conjugated anti-rabbit at 1/10000 dilution
Immunohistochemistry analysis in formalin fixed and paraffin embedded Human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107846 at a dilution of 1/50.
References for Anti-ITIH5 antibody (ab107846)
has not yet been referenced specifically in any publications.
Publishing research using ab107846? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"