Overview

  • Product name
  • Description
    Rabbit polyclonal to ITIH5
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, between amino acids 578-606 from the C terminal region of Human ITIH5 (NP_085046.4, NP_116206.3, NP_001001851.1).

  • Positive control
    • NCI-H460 cell line lysate, Human breast carcinoma tissue IF/ICC: JEG3 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab107846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 105 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May act as a tumor suppressor.
  • Tissue specificity
    Abundantly expressed in placenta. Less abundant expression in mammary gland and ovary. Expression is barely detectable levels in all other tissues tested.
  • Sequence similarities
    Belongs to the ITIH family.
    Contains 1 VIT domain.
    Contains 1 VWFA domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Inter-alpha-inhibitor heavy chain 5 antibody
    • Inter-alpha-trypsin inhibitor heavy chain H5 antibody
    • ITI heavy chain H5 antibody
    • ITI-HC5 antibody
    • ITIH5 antibody
    • ITIH5_HUMAN antibody
    • KIAA1953 antibody
    • PP14776 antibody
    • UNQ311/PRO354 antibody
    see all

Images

  • ab107846 stained JEG3 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab107846 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-ITIH5 antibody (ab107846) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Secondary
    HRP conjugated anti-rabbit at 1/10000 dilution

    Predicted band size : 105 kDa
  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107846 at a dilution of 1/50.

References

ab107846 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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