Overview

  • Product nameITK antibody
  • Host speciesRabbit
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein:

    MNNFILLEEQ LIKKSQQKRR TSPSNFKVRF FVLTKASLAY FEDRHGKKRT LKGSIELSRI KCVEIVKSDI SIPCHYKYPF QVVHDNYLLY VFAPDRESRQ RWVLALKEET RNNNSLVPKY HPNFWMDGKW RCCSQLEKLA TGCAQYDPTK NASKKPLPPT PEDNRRPLWE PEETVVIALY DYQTNDPQEL ALRRNEEYCL LDSSEIHWWR VQDRNGHEGY VPSSYLVEKS PNNLETYEWY NKSISRDKAE KLLLDTGKEG AFMVRDSRTA

    , corresponding to amino acids 1-270 of Human ITK. spanning the PH, SH3 domains and a small portion of the SH2 domain.

  • Positive controlJurkat cell lysate.
  • General notes

    Raised in: Rabbit

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4
  • Concentration information loading...
  • IsotypeIgG
  • Research areas
  • FunctionPlays a role in T-cell proliferation and differentiation.
  • Tissue specificityT-cell lines and natural killer cell lines.
  • Involvement in diseaseDefects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localizationCell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
  • Information by UniProt
  • Alternative names
    • EMT
    • Homolog of mouse T cell itk/tsk
    • IL 2 inducible T cell kinase
    • IL2 inducible T cell kinase
    • Interleukin 2 inducible T cell kinase
    • Itk
    • ITK_HUMAN
    • Kinase EMT
    • LPFS1
    • LYK
    • MGC126257
    • MGC126258
    • PSCTK 2
    • PSCTK2
    • T cell specific kinase
    • T-cell-specific kinase
    • TSK
    • Tyrosine protein kinase
    • Tyrosine protein kinase ITK/TSK
    • Tyrosine protein kinase Lyk
    • Tyrosine-protein kinase ITK/TSK
    • Tyrosine-protein kinase Lyk
    see all
  • Database links
  • Cellular localizationCell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.

Applications

Our Abpromise guarantee covers the use of ab14510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 mg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).
IP Use at an assay dependent dilution.

References for ITK antibody (ab14510)

ab14510 has not yet been referenced specifically in any publications.

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