Overview

  • Product name
  • Description
    Rabbit polyclonal to ITK
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH selected from a sequence within amino acids 100-200 of human ITK.

  • Positive control
    • CEM cell lysate IF/ICC: HeLa cell line

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 72 kDa).
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.

Target

  • Function
    Plays a role in T-cell proliferation and differentiation.
  • Tissue specificity
    T-cell lines and natural killer cell lines.
  • Involvement in disease
    Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Cellular localization
    Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • EMT antibody
    • Homolog of mouse T cell itk/tsk antibody
    • IL 2 inducible T cell kinase antibody
    • IL2 inducible T cell kinase antibody
    • Interleukin 2 inducible T cell kinase antibody
    • Itk antibody
    • ITK_HUMAN antibody
    • Kinase EMT antibody
    • LPFS1 antibody
    • LYK antibody
    • MGC126257 antibody
    • MGC126258 antibody
    • PSCTK 2 antibody
    • PSCTK2 antibody
    • T cell specific kinase antibody
    • T-cell-specific kinase antibody
    • TSK antibody
    • Tyrosine protein kinase antibody
    • Tyrosine protein kinase ITK/TSK antibody
    • Tyrosine protein kinase Lyk antibody
    • Tyrosine-protein kinase ITK/TSK antibody
    • Tyrosine-protein kinase Lyk antibody
    see all

Images

  • ICC/IF image of ab37826 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37826, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-ITK antibody (ab37826) at 1/100 dilution + CEM cell lysate

    Predicted band size : 72 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)

References

ab37826 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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