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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Tec Family
Overview
Product name
Anti-ITK antibody
See all ITK products (6) ...
Description
Goat polyclonal to ITK
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide: RLLRQLAEIAESGL, corresponding to C terminal amino acids 607-620 of Human ITK.
C-RLLRQLAE IAESGL
Positive control
Jurkat cell lysate.
General notes
GenBank Accession Number – NP_005537
LocusLink ID - 3702
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Tec Family
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Western blot - ITK antibody (ab9707)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
Western Blot – Approx 75 kDa bands seen in Jurkat Lysates. Recommended for use at 0.5-2 µg/ml.
Target
Function
Plays a role in T-cell proliferation and differentiation.
Tissue specificity
T-cell lines and natural killer cell lines.
Involvement in disease
Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Cellular localization
Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
Target information above from: UniProt accessionQ08881
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EMT antibody
- Homolog of mouse T cell itk/tsk antibody
- IL2 inducible T cell kinase antibody
see all
Database links
- Entrez Gene: 3702 Human
- Entrez Gene: 16428 Mouse
- Omim: 186973 Human
- SwissProt: Q08881 Human
- SwissProt: Q32ML7 Human
- SwissProt: Q03526 Mouse
- SwissProt: Q3U5G1 Mouse
- SwissProt: Q5STT7 Mouse
see all
Anti-ITK antibody images:
Western blot - ITK antibody (ab9707)
ab9707 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
References for Anti-ITK antibody (ab9707)
This product has been referenced in:
- Douhan Jet al. A FLIPR-based assay to assess potency and selectivity of inhibitors of the TEC family kinases Btk and Itk. Assay Drug Dev Technol 5:751-8 (2007). WB; Human.Read more (PubMed: 18181691) »
See 1 publication for this product
Publishing research using ab9707? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"