Overview

  • Product name
  • Description
    Goat polyclonal to ITK
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide: RLLRQLAEIAESGL, corresponding to C terminal amino acids 607-620 of Human ITK.

  • Positive control
    • Jurkat cell lysate.
  • General notes
    GenBank Accession Number – NP_005537 LocusLink ID - 3702

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Western Blot – Approx 75 kDa bands seen in Jurkat Lysates. Recommended for use at 0.5-2 µg/ml.
  • Target

    • Function
      Plays a role in T-cell proliferation and differentiation.
    • Tissue specificity
      T-cell lines and natural killer cell lines.
    • Involvement in disease
      Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
      Contains 1 Btk-type zinc finger.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • Cellular localization
      Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • EMT antibody
      • Homolog of mouse T cell itk/tsk antibody
      • IL 2 inducible T cell kinase antibody
      • IL2 inducible T cell kinase antibody
      • Interleukin 2 inducible T cell kinase antibody
      • Itk antibody
      • ITK_HUMAN antibody
      • Kinase EMT antibody
      • LPFS1 antibody
      • LYK antibody
      • MGC126257 antibody
      • MGC126258 antibody
      • PSCTK 2 antibody
      • PSCTK2 antibody
      • T cell specific kinase antibody
      • T-cell-specific kinase antibody
      • TSK antibody
      • Tyrosine protein kinase antibody
      • Tyrosine protein kinase ITK/TSK antibody
      • Tyrosine protein kinase Lyk antibody
      • Tyrosine-protein kinase ITK/TSK antibody
      • Tyrosine-protein kinase Lyk antibody
      see all

    Images

    • ab9707 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

    References

    This product has been referenced in:
    • Douhan J  et al. A FLIPR-based assay to assess potency and selectivity of inhibitors of the TEC family kinases Btk and Itk. Assay Drug Dev Technol 5:751-8 (2007). WB ; Human . Read more (PubMed: 18181691) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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