• Product nameAnti-ITM2B antibody
    See all ITM2B primary antibodies
  • Description
    Mouse polyclonal to ITM2B
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1 - 266 of Human ITM2B (NP_068839.1)

  • Positive control
    • HepG2 cell lysate



Our Abpromise guarantee covers the use of ab89352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.


  • FunctionFunctions as a protease inhibitor. Plays a role in APP processing regulating the physiological production of the beta amyloid peptide. Restricts docking of gamma-secretase to APP and access of alpha- and beta-secretase to their cleavage APP sequence.
  • Tissue specificityExpressed in brain and in other tissues.
  • Involvement in diseaseDefects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 1 (CAA-ITM2B1) [MIM:176500]. A disorder characterized by amyloid deposition in the walls of cerebral blood vessels and neurodegeneration in the central nervous system. Cerebral amyloid angiopathy, non-neuritic and perivascular plaques and neurofibrillary tangles are the predominant pathological lesions. Clinical features include progressive mental deterioration, spasticity and muscular rigidity.
    Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 2 (CAA-ITM2B2) [MIM:117300]; also known as heredopathia ophthalmo-oto-encephalica. A disorder characterized by amyloid deposition in the walls of the blood vessels of the cerebrum, choroid plexus, cerebellum, spinal cord and retina. Plaques and neurofibrillary tangles are observed in the hippocampus. Clinical features include progressive ataxia, dementia, cataracts and deafness.
  • Sequence similaritiesBelongs to the ITM2 family.
    Contains 1 BRICHOS domain.
  • Post-translational
    The C-terminal part of the ectodomain is processed by furin and related proteases producing a secreted peptide of 4 to 5 kDa. For the ABRI and ADAN variants the C-terminal secreted peptide is larger and may produce amyloid fibrils responsible for neuronal dysfunction and dementia. The remaining part of the ectodomain containing the BRICHOS domain is cleaved by ADAM10 and is secreted as a peptide of 25 kDa. The membrane-bound N-terminal fragment (NTF) of 22 kDa is further proteolytically processed by SPPL2A and SPPL2B through regulated intramembrane proteolysis producing a secreted peptide (BRI2C) and an intracellular domain (ICD) released in the cytosol.
  • Cellular localizationGolgi apparatus membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABRI antibody
    • ABri/ADan amyloid peptide antibody
    • BRI 2 antibody
    • BRI antibody
    • BRI2 antibody
    • BRICD 2B antibody
    • BRICD2B antibody
    • BRICHOS domain containing 2B antibody
    • E25B antibody
    • E3 16 antibody
    • E3-16 antibody
    • FBD antibody
    • Integral membrane protein 2B antibody
    • ITM 2B antibody
    • ITM2B antibody
    • ITM2B_HUMAN antibody
    • Protein E25B antibody
    • Transmembrane protein BRI antibody
    see all

Anti-ITM2B antibody images

  • Anti-ITM2B antibody (ab89352) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 30 kDa
  • All lanes : Anti-ITM2B antibody (ab89352) at 1 µg/ml

    Lane 1 : ITM2B transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 30 kDa
  • IHC-P image of ITM2B antibody (ab89352) on small bowel tissue. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 3% H2O2 solution for 10 minutes at 22°C.

    See Abreview

References for Anti-ITM2B antibody (ab89352)

ab89352 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Small Bowel)
Specification Small Bowel
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 22°C

Dr. Aamir Ahmed

Verified customer

Submitted Jan 27 2011