Overview

  • Product name
    Anti-ITM2B antibody [OTI1C11]
    See all ITM2B primary antibodies
  • Description
    Mouse monoclonal [OTI1C11] to ITM2B
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human ITM2B produced in HEK293T cells (NP_068839).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY ITM2B cDNA; Human kidney tissue; HeLa and Jurkat cells.
  • General notes

    Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

    The clone number has been updated from 1C11 to OTI1C11, both clone numbers name the same clone.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab119044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 30 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Functions as a protease inhibitor. Plays a role in APP processing regulating the physiological production of the beta amyloid peptide. Restricts docking of gamma-secretase to APP and access of alpha- and beta-secretase to their cleavage APP sequence.
  • Tissue specificity
    Expressed in brain and in other tissues.
  • Involvement in disease
    Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 1 (CAA-ITM2B1) [MIM:176500]. A disorder characterized by amyloid deposition in the walls of cerebral blood vessels and neurodegeneration in the central nervous system. Cerebral amyloid angiopathy, non-neuritic and perivascular plaques and neurofibrillary tangles are the predominant pathological lesions. Clinical features include progressive mental deterioration, spasticity and muscular rigidity.
    Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 2 (CAA-ITM2B2) [MIM:117300]; also known as heredopathia ophthalmo-oto-encephalica. A disorder characterized by amyloid deposition in the walls of the blood vessels of the cerebrum, choroid plexus, cerebellum, spinal cord and retina. Plaques and neurofibrillary tangles are observed in the hippocampus. Clinical features include progressive ataxia, dementia, cataracts and deafness.
  • Sequence similarities
    Belongs to the ITM2 family.
    Contains 1 BRICHOS domain.
  • Post-translational
    modifications
    The C-terminal part of the ectodomain is processed by furin and related proteases producing a secreted peptide of 4 to 5 kDa. For the ABRI and ADAN variants the C-terminal secreted peptide is larger and may produce amyloid fibrils responsible for neuronal dysfunction and dementia. The remaining part of the ectodomain containing the BRICHOS domain is cleaved by ADAM10 and is secreted as a peptide of 25 kDa. The membrane-bound N-terminal fragment (NTF) of 22 kDa is further proteolytically processed by SPPL2A and SPPL2B through regulated intramembrane proteolysis producing a secreted peptide (BRI2C) and an intracellular domain (ICD) released in the cytosol.
  • Cellular localization
    Golgi apparatus membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABRI antibody
    • ABri/ADan amyloid peptide antibody
    • BRI 2 antibody
    • BRI antibody
    • BRI2 antibody
    • BRICD 2B antibody
    • BRICD2B antibody
    • BRICHOS domain containing 2B antibody
    • E25B antibody
    • E3 16 antibody
    • E3-16 antibody
    • FBD antibody
    • imBRI2 antibody
    • Immature BRI2 antibody
    • Integral membrane protein 2B antibody
    • ITM 2B antibody
    • ITM2B antibody
    • ITM2B_HUMAN antibody
    • Protein E25B antibody
    • RDGCA antibody
    • Transmembrane protein BRI antibody
    see all

Images

  • All lanes : Anti-ITM2B antibody [OTI1C11] (ab119044) at 1/2000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY ITM2B cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 30 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202377)
  • ab119044, at 1/150 dilution, staining ITM2B in paraffin embedded Human kidney by Immunohistochemistry.
  • ab119044 at 1/100 dilution staining ITM2B in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab119044 at 1/100 dilution staining ITM2B in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References

ab119044 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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