Overview

  • Product name
    Anti-ITM2C antibody
  • Description
    Rabbit polyclonal to ITM2C
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 110 and 258 of ITM2C (AAH02424).

  • Positive control
    • HeLa, HepG2, MCF7 cell lines.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer or Tris-EDTA buffer (PH8.0)

Target

  • Function
    Negative regulator of beta amyloid peptide production. May inhibit the processing of APP by blocking its access to alpha-and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation.
  • Tissue specificity
    High levels in the brain, specifically in the cerebral cortex, medulla, amygdala, hippocampus, thalamus, caudate nucleus, cerebellum, olfactory lobe and spinal cord. Very low levels in other organs.
  • Sequence similarities
    Belongs to the ITM2 family.
    Contains 1 BRICHOS domain.
  • Post-translational
    modifications
    Type I membrane-bound, as well as soluble, furin has a pre-eminent role in ITM2C proteolytic processing. PCSK7 and PCSK5 may also be involved although to a lesser extent. The soluble form of PCSK7 is incapable of processing ITM2C.
  • Cellular localization
    Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRI3 antibody
    • BRICD2C antibody
    • BRICHOS domain containing 2C antibody
    • Cerebral protein 14 antibody
    • CT-BRI3 antibody
    • E25 antibody
    • E25C antibody
    • Integral membrane protein 2C antibody
    • Integral membrane protein 3 antibody
    • Itm2c antibody
    • ITM2C_HUMAN antibody
    • ITM3 antibody
    • NPD018 antibody
    • Transmembrane protein BRI3 antibody
    see all

Images

  • Anti-ITM2C antibody (ab101389) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 30 kDa
  • Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ab101389 at 1/100 dilution.

References

ab101389 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Immuno-precipitation step
Other - Dynabeads
Sample
Human Cell lysate - whole cell (SH-SY5Y)
Specification
SH-SY5Y
Total protein in input
1000 µg
Username

Ms. Filipa Martins

Verified customer

Submitted Jun 20 2014

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (5-20% gradient)
Sample
Rat Tissue lysate - whole (Cortex)
Specification
Cortex
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Filipa Martins

Verified customer

Submitted Nov 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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