Recombinant
RabMAb

Anti-ITPK1 antibody [EPR10631] (ab175222)

Overview

  • Product name
    Anti-ITPK1 antibody [EPR10631]
    See all ITPK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10631] to ITPK1
  • Tested applications
    Suitable for: IHC-P, WB, IP, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ITPK1 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13572

  • Positive control
    • Fetal brain, fetal heart, HepG2 and Hela lysates; Human brain and liver tissues; Hela cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10631
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/10000 - 1/50000. Predicted molecular weight: 46 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetraphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain.
    • Tissue specificity
      Expressed in brain > heart > skeletal muscle = kidney = pancreas = liver = placenta > lung. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal cord, occipital lobe, frontal lobe, temporal lobe and putamen.
    • Sequence similarities
      Belongs to the ITPK1 family.
      Contains 1 ATP-grasp domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3 antibody
      • 4)P(3) 5/6-kinase antibody
      • 4-trisphosphate 5/6-kinase antibody
      • Inositol 1 3 4 triphosphate 5/6 kinase antibody
      • Inositol 1 3 4 trisphosphate 5/6 kinase antibody
      • Inositol 1 antibody
      • Inositol tetrakisphosphate 1 kinase antibody
      • Inositol triphosphate 5/6 kinase antibody
      • Inositol-tetrakisphosphate 1-kinase antibody
      • Inositol-triphosphate 5/6-kinase antibody
      • Ins(1 3 4)P(3) 5/6 kinase antibody
      • Ins(1 antibody
      • ITPK 1 antibody
      • itpk1 antibody
      • ITPK1_HUMAN antibody
      • ITRPK 1 antibody
      • ITRPK1 antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175222 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Flow cytometric analysis of permeabilized HeLa cells labeling ITPK1 with ab175222 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITPK1 with ab175222 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ITPK1 with ab175222 at 1/50 dilution.

    • All lanes : Anti-ITPK1 antibody [EPR10631] (ab175222) at 1/10000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Hela cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 46 kDa

    References

    ab175222 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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