Recombinant Anti-ITPK1 antibody [EPR10631] (ab175222)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10631] to ITPK1
- Suitable for: IHC-P, WB, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ITPK1 antibody [EPR10631]
See all ITPK1 primary antibodies -
Description
Rabbit monoclonal [EPR10631] to ITPK1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IP, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal brain, fetal heart, HepG2 and Hela lysates; Human brain and liver tissues; Hela cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10631 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/10000 - 1/50000. Predicted molecular weight: 46 kDa.
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IP |
1/10 - 1/100.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Predicted molecular weight: 46 kDa. |
IP
1/10 - 1/100. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetraphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain. -
Tissue specificity
Expressed in brain > heart > skeletal muscle = kidney = pancreas = liver = placenta > lung. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal cord, occipital lobe, frontal lobe, temporal lobe and putamen. -
Sequence similarities
Belongs to the ITPK1 family.
Contains 1 ATP-grasp domain. - Information by UniProt
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Database links
- Entrez Gene: 3705 Human
- Omim: 601838 Human
- SwissProt: Q13572 Human
- Unigene: 308122 Human
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Alternative names
- 3 antibody
- 4)P(3) 5/6-kinase antibody
- 4-trisphosphate 5/6-kinase antibody
see all
Images
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Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175222 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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Intracellular flow cytometric analysis of permeabilized HeLa cellslabeling ITPK1 with ab175222 at 1/10 dilution (red) compared toa rabbit IgG negative control (green).
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITPK1 with ab175222 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ITPK1 with ab175222 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-ITPK1 antibody [EPR10631] (ab175222) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Fetal liver lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Hela cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab175222 has been referenced in 1 publication.
- Desfougères Y et al. ITPK1 mediates the lipid-independent synthesis of inositol phosphates controlled by metabolism. Proc Natl Acad Sci U S A 116:24551-24561 (2019). PubMed: 31754032