The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 308 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Use at an assay dependent dilution.
FunctionReceptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release is regulated by cAMP both dependently and independently of PKA.
Tissue specificityIsoform Short is found in skeletal muscle and heart.
Involvement in diseaseAnhidrosis, isolated, with normal sweat glands
Sequence similaritiesBelongs to the InsP3 receptor family. Contains 5 MIR domains.
DomainThe receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
Post-translational modificationsPhosphorylation by cAMP-dependent PKA on Ser-937 increases calcium release.