Anti-IWS1 antibody - N-terminal (ab195309)
Key features and details
- Rabbit polyclonal to IWS1 - N-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IWS1 antibody - N-terminal
See all IWS1 primary antibodies -
Description
Rabbit polyclonal to IWS1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Goat, Cow, Dog, Pig, Saccharomyces cerevisiae, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Bat -
Immunogen
Synthetic peptide within Human IWS1 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q96ST2 -
Positive control
- HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 92 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/1000 - 1/5000. Predicted molecular weight: 92 kDa. |
Target
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Sequence similarities
Belongs to the IWS1 family.
Contains 1 TFIIS N-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55677 Human
- Entrez Gene: 73473 Mouse
- Entrez Gene: 291705 Rat
- SwissProt: Q96ST2 Human
- SwissProt: Q8C1D8 Mouse
- SwissProt: Q3SWT4 Rat
- Unigene: 469879 Human
- Unigene: 66853 Mouse
see all -
Alternative names
- DKFZp761G0123 antibody
- FLJ10006 antibody
- FLJ14655 antibody
see all
Images
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Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: ab195309 used for IP at 6 µg per reaction. For blotting immunoprecipitated IWS1, ab195309 was used at 0.4 µg/mL.
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All lanes : Anti-IWS1 antibody - N-terminal (ab195309) at 0.4 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Mouse TCMK-1 cell lysate
Lane 5 : Mouse NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 92 kDa
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab195309 has not yet been referenced specifically in any publications.