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- Secondary antibodies
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Product name
Anti-JAB1 antibody
See all JAB1 products (12) ...
Description
Rabbit polyclonal to JAB1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Zebrafish
Immunogen
Immunogen was a synthetic peptide, which represented a c-terminal domain of human JAB1 (LocusLink ID 10987).
Positive control
HEK 293T cells.
General notes
Centrifuge tube to remove product from lid.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified using the peptide immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Deubiquitination
Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Regulation
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Proteasome
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
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Western blot - JAB1 antibody (ab12323)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/25000. Detects a band of approximately 33, 36 kDa (predicted molecular weight: 40 kDa).
Target
Function
Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.
Sequence similarities
Belongs to the peptidase M67A family. CSN5 subfamily.
Contains 1 MPN (JAB/Mov34) domain.
Domain
The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ92905
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 38 kDa Mov34 homolog antibody
- 38 kDa MOV34 isologue antibody
- COP9 (constitutive photomorphogenic) homolog subunit 5 antibody
see all
Database links
- Entrez Gene: 10987 Human
- Entrez Gene: 26754 Mouse
- Entrez Gene: 393698 Zebrafish
- Omim: 604850 Human
- SwissProt: Q92905 Human
- SwissProt: O35864 Mouse
- SwissProt: Q6PC30 Zebrafish
- Unigene: 491912 Human
see all
Anti-JAB1 antibody images:
Western blot - JAB1 antibody (ab12323)
Predicted band size : 40 kDa
Detection of Human CSN5 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody: Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
References for Anti-JAB1 antibody (ab12323)
This product has been referenced in:
- Chua YS et al. Regulation of cullin RING E3 ubiquitin ligases by CAND1 in vivo. PLoS One 6:e16071 (2011). WB; Human.Read more (PubMed: 21249194) »
- Leck YC et al. Biochemical and cellular effects of inhibiting Nedd8 conjugation. Biochem Biophys Res Commun 398:588-93 (2010). WB; Human.Read more (PubMed: 20603103) »
See all 3 publications for this product
Publishing research using ab12323? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"