Overview

  • Product nameAnti-JAB1 antibody [2A10]
    See all JAB1 primary antibodies
  • Description
    Mouse monoclonal [2A10] to JAB1
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein encoding the full-length (amino acids 1-334) mouse JAB-1 gene expressed in E. coli.

  • Positive control
    • Recombinant JAB-1 protein, 293 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml.
IP Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionProbable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.
  • Sequence similaritiesBelongs to the peptidase M67A family. CSN5 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.
  • DomainThe JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 38 kDa Mov34 homolog antibody
    • COP9 (constitutive photomorphogenic) homolog subunit 5 antibody
    • COP9 constitutive photomorphogenic homolog subunit 5 antibody
    • COP9 signalosome complex subunit 5 antibody
    • COP9 signalosome subunit 5 antibody
    • Cop9 subunit 5 antibody
    • COPS 5 antibody
    • cops5 antibody
    • CSN 5 antibody
    • CSN5 antibody
    • CSN5_HUMAN antibody
    • JAB 1 antibody
    • Jun activation domain binding protein 1 antibody
    • Jun activation domain binding protein antibody
    • Jun activation domain-binding protein 1 antibody
    • MGC3149 antibody
    • MOV 34 antibody
    • MOV34 antibody
    • MOV34 family, 38-KD member antibody
    • SGN 5 antibody
    • SGN5 antibody
    • Signalosome subunit 5 antibody
    see all

Anti-JAB1 antibody [2A10] images

  • IHC image of ab495 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab495, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemical analysis of rat testis tissue, staining JAB1 with ab495. Sections were incubated with primary antibody overnight at 4°C. A rhodamine-conjugated goat anti-mouse IgG was used as the secondary antibody.

References for Anti-JAB1 antibody [2A10] (ab495)

This product has been referenced in:
  • Füzesi-Levi MG  et al. Dynamic regulation of the COP9 signalosome in response to DNA damage. Mol Cell Biol 34:1066-76 (2014). WB ; Human . Read more (PubMed: 24421388) »
  • Turtzo LC  et al. Deletion of macrophage migration inhibitory factor worsens stroke outcome in female mice. Neurobiol Dis 54:421-31 (2013). WB ; Mouse . Read more (PubMed: 23376686) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (OBL21 cells)
Loading amount 30 µg
Specification OBL21 cells
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted Apr 08 2009

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (OBL21 cells)
Specification OBL21 cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% saponin
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

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Verified customer

Submitted Apr 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"