• Product nameAnti-JAB1 antibody [EPR1350]
    See all JAB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1350] to JAB1
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human JAB1.

  • Positive control
    • NIH 3T3, U87-MG, MCF7, and PC12 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab124720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionProbable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.
    • Sequence similaritiesBelongs to the peptidase M67A family. CSN5 subfamily.
      Contains 1 MPN (JAB/Mov34) domain.
    • DomainThe JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 38 kDa Mov34 homolog antibody
      • COP9 (constitutive photomorphogenic) homolog subunit 5 antibody
      • COP9 constitutive photomorphogenic homolog subunit 5 antibody
      • COP9 signalosome complex subunit 5 antibody
      • COP9 signalosome subunit 5 antibody
      • Cop9 subunit 5 antibody
      • COPS 5 antibody
      • cops5 antibody
      • CSN 5 antibody
      • CSN5 antibody
      • CSN5_HUMAN antibody
      • JAB 1 antibody
      • Jun activation domain binding protein 1 antibody
      • Jun activation domain binding protein antibody
      • Jun activation domain-binding protein 1 antibody
      • MGC3149 antibody
      • MOV 34 antibody
      • MOV34 antibody
      • MOV34 family, 38-KD member antibody
      • SGN 5 antibody
      • SGN5 antibody
      • Signalosome subunit 5 antibody
      see all

    Anti-JAB1 antibody [EPR1350] images

    • All lanes : Anti-JAB1 antibody [EPR1350] (ab124720) at 1/1000 dilution

      Lane 1 : NIH 3T3 cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : PC12 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 38 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-JAB1 antibody [EPR1350] (ab124720)

    This product has been referenced in:
    • Wang R  et al. COPS5 protein overexpression increases amyloid plaque burden, decreases spinophilin-immunoreactive puncta, and exacerbates learning and memory deficits in the mouse brain. J Biol Chem 290:9299-309 (2015). Mouse . Read more (PubMed: 25713139) »
    • Wang H  et al. COPS5 (Jab1) protein increases ß site processing of amyloid precursor protein and amyloid ß peptide generation by stabilizing RanBP9 protein levels. J Biol Chem 288:26668-77 (2013). Read more (PubMed: 23926111) »

    See all 2 Publications for this product

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