The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
Jagged 2 is a Notch ligand involved in the mediation of Notch signaling. Jagged 2 is expressed in heart, placenta and skeletal muscle and to a lesser extend in pancreas. Very low expression in brain, lung, liver and kidney. The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. Jagged 2 is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for Jagged 2.
ab60041 at 1/50-1/100 dilution staining Jagged 2 in Human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Shi FT et al. Notch signaling is significantly suppressed in basal cell carcinomas and activation induces basal cell carcinoma cell apoptosis. Mol Med Rep15:1441-1454 (2017).
Read more (PubMed: 28259916) »
Pietras A et al. JAG2 Induction in Hypoxic Tumor Cells Alters Notch Signaling and Enhances Endothelial Cell Tube Formation. Mol Cancer Res9:626-36 (2011).
Read more (PubMed: 21402725) »