Overview

  • Product nameAnti-Jagged 2 antibody
    See all Jagged 2 primary antibodies
  • Description
    Rabbit polyclonal to Jagged 2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide derived from a region within N terminal amino acids 1-50 (MRAQGRGRLP RRLLLLLALW VQAARPMGYF ELQLSALRNV NGELLSGACC) of Human Jagged 2

  • Positive control
    • Jurkat membrane lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 133 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceJagged 2 is a Notch ligand involved in the mediation of Notch signaling. Jagged 2 is expressed in heart, placenta and skeletal muscle and to a lesser extend in pancreas. Very low expression in brain, lung, liver and kidney. The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. Jagged 2 is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for Jagged 2.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • D12Ggc2e antibody
    • HJ 2 antibody
    • HJ2 antibody
    • JAG 2 antibody
    • JAG2 antibody
    • Jagged 2 precursor antibody
    • Jagged2 antibody
    • Protein jagged 2 antibody
    • SER2 antibody
    • Syndactylism antibody
    see all

Anti-Jagged 2 antibody images

  • Anti-Jagged 2 antibody (ab80671) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 133 kDa
    Observed band size : 133 kDa
    Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Jagged 2 antibody (ab80671)

ab80671 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Embryos -GD9)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 20 µg
Treatment hydroxyurea
Specification Embryos -GD9
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Nazem El Husseini

Verified customer

Submitted May 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"