Overview

  • Product nameAnti-JAK1 antibody
    See all JAK1 primary antibodies
  • Description
    Rabbit polyclonal to JAK1
  • SpecificityDetects endogenous levels of JAK1 protein
  • Tested applicationsSuitable for: ICC/IF, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic non - phosphopeptide (Human) from around the phosphorylation site of Tyrosine 1022

  • Positive control
    • Human breast carcinoma tissue. Extracts from MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab47435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).

Target

  • FunctionTyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
  • Tissue specificityExpressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • DomainPossesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
    The FERM domain mediates interaction with JAKMIP1.
  • Cellular localizationEndomembrane system. Wholly intracellular, possibly membrane associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • JAK 1 antibody
    • JAK 1A antibody
    • JAK 1B antibody
    • JAK-1 antibody
    • JAK1 antibody
    • JAK1_HUMAN antibody
    • JAK1A antibody
    • JAK1B antibody
    • Janus kinase 1 (a protein tyrosine kinase) antibody
    • Janus kinase 1 antibody
    • JTK3 antibody
    • Tyrosine protein kinase JAK 1 antibody
    • Tyrosine protein kinase JAK1 antibody
    • Tyrosine-protein kinase JAK1 antibody
    see all

Anti-JAK1 antibody images

  • Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab47435 at dilution of 1/50-1/100. Right panel treated with peptide and left panel untreated
  • All lanes : Anti-JAK1 antibody (ab47435) at 1/500 dilution

    Lane 1 : MCF7 cell extract treated with EGF
    Lane 2 : MCF7 cell extract treated with EGF with peptide


    Predicted band size : 132 kDa
  • ICC/IF image of ab47435 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47435, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-JAK1 antibody (ab47435)

This product has been referenced in:
  • Murphy MJ  et al. Leukemia Inhibitory Factor is Necessary for Ovulation in Female Rhesus Macaques. Endocrinology N/A:en20161283 (2016). IHC ; Macaque Monkey . Read more (PubMed: 27571132) »
  • El Jamal SM  et al. Interferon gamma-induced apoptosis of head and neck squamous cell carcinoma is connected to indoleamine-2,3-dioxygenase via mitochondrial and ER stress-associated pathways. Cell Div 11:11 (2016). WB ; Human . Read more (PubMed: 27486476) »

See all 3 Publications for this product

Product Wall

Please accept my apologies for you having trouble with ab47435. The anti-JAK1 antibody is covered under our Abpromise, which states that we guarantee all of our antibodies for 6 months to work as stated on the datasheet. In the case of ab47435, it is g...

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