Overview

  • Product nameAnti-JAK3 antibody [EPR908(2)]
    See all JAK3 primary antibodies
  • Description
    Rabbit monoclonal [EPR908(2)] to JAK3
  • Tested applicationsSuitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues on the N-terminus in Human JAK3

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab92595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
  • Application notesICC: 1/250 - 1/500.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 125 kDa.

    Is unsuitable for Flow Cyt or IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.
    • Tissue specificityIn NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.
    • Involvement in diseaseDefects in JAK3 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-negative (T(-)B(+)NK(-) SCID) [MIM:600802]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
    • DomainPossesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
    • Post-translational
      modifications
      Tyrosine phosphorylated in response to IL-2 and IL-4.
    • Cellular localizationEndomembrane system. Wholly intracellular, possibly membrane associated.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.10.2 antibody
      • JAK 3 antibody
      • JAK L antibody
      • JAK-3 antibody
      • Jak3 antibody
      • JAK3 HUMAN antibody
      • JAK3_HUMAN antibody
      • JAKL antibody
      • Janus kinase 3 (a protein tyrosine kinase, leukocyte) antibody
      • Janus kinase 3 antibody
      • Janus Kinase3 antibody
      • L JAK antibody
      • L-JAK antibody
      • Leukocyte janus kinase antibody
      • LJAK antibody
      • Protein tyrosine kinase leukocyte antibody
      • Tyrosine protein kinase JAK3 antibody
      • Tyrosine-protein kinase JAK3 antibody
      see all

    Anti-JAK3 antibody [EPR908(2)] images

    • All lanes : Anti-JAK3 antibody [EPR908(2)] (ab92595) at 1/1000 dilution

      Lane 1 : IM-9 cell lysate
      Lane 2 : TF-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 125 kDa

    References for Anti-JAK3 antibody [EPR908(2)] (ab92595)

    ab92595 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92595.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"