The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
FunctionMay function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells. Enhances myeloid leukemia cell adhesion to endothelial cells.
Tissue specificityExpression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus.
Sequence similaritiesBelongs to the immunoglobulin superfamily. Contains 2 Ig-like V-type (immunoglobulin-like) domains.
DomainThe Ig-like V-type domain 2 mediates interaction with CXADR.
Cellular localizationCell junction. Cell membrane. Localized at the plasma membrane in areas of cell-cell contacts and at the cell surface of PMN.