Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)

Overview

  • Product name
    Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1]
  • Description
    Mouse monoclonal [JN1] to Japanese encephalitis virus NS1 glycoprotein
  • Tested applications
    Suitable for: Flow Cyt, IHC-FoFr, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Japanese encephalitis virus
  • Immunogen

    Full length native protein purified from Japanese encephalitis virus (Nakayama) supernatant

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 10mM Sodium Azide
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    JN1
  • Isotype
    IgG3
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 20581148

ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration. PubMed: 19635909
WB 1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 46 kDa.
ICC/IF 1/5 - 1/20.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    The Japanese encephalitis viral genome encodes 7 non-structural proteins NS1-NS5. NS1 contains N-linked carbohydrate chains at positions 130 and 207. It is not incorporated into the virion but exists in the host cell, on the cell surface and can also be extracellular.
  • Database links
    • Alternative names
      • Japanese encephalitis non-structural protein 1 antibody
      • Japanese encephalitis NS1 antibody
      • Japanese encephalitis virus non-structural protein 1 antibody

    Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] images

    • Japanese encephalitis virus (Nakayama) infected PS clone D cells stained with ab41651 (green).
    • All lanes : Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)

      Lane 1 : Japanese encephalitis virus infected C6/36 cell lysate (unheated)
      Lane 2 : Japanese encephalitis virus infected C6/36 cell lysate (boiled)


      Predicted band size : 46 kDa
      Observed band size : 46,92 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa (possible cleavage fragment),50 kDa (possible cleavage fragment).This antibody recognsies 2 forms of NS1 - NS1 and NS1' (46 and 53 kDa respectively). NS1' is thought to be formed when NS1 is cleaved from NS2A at an alternative site. Both NS1 and NS1' exist as dimers in untreated samples but are dissociated into monomers when samples are boiled.

    References for Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)

    This product has been referenced in:
    • Sharma N  et al. Japanese Encephalitis Virus exploits the microRNA-432 to regulate the expression of Suppressor of Cytokine Signaling (SOCS) 5. Sci Rep 6:27685 (2016). WB ; Japanese encephalitis virus . Read more (PubMed: 27282499) »
    • Sharma N  et al. miR-146a suppresses cellular immune response during Japanese encephalitis virus JaOArS982 strain infection in human microglial cells. J Neuroinflammation 12:30 (2015). WB ; Japanese encephalitis virus . Read more (PubMed: 25889446) »

    See all 6 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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