Overview

  • Product name
    Anti-Jarid2 antibody
    See all Jarid2 primary antibodies
  • Description
    Rabbit polyclonal to Jarid2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human Jarid2 aa 662-891. The exact sequence is proprietary.
    Database link: Q92833

  • Positive control
    • Jarid2-transfected 293T whole cell lysate and Mouse fetal forebrain lysate; Jarid2-transfected HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab184152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Predicted molecular weight: 138 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.
  • Tissue specificity
    During embryogenesis, predominantly expressed in neurons and particularly in dorsal root ganglion cells.
  • Sequence similarities
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
  • Domain
    The ARID domain is required to target the PRC2 complex to its target genes.
    The GSGFP motif is required for the interaction with SUZ12.
  • Cellular localization
    Nucleus. Colocalizes with the PRC2 complex on chromatin.
  • Information by UniProt
  • Database links
    • Alternative names
      • JARD2 antibody
      • JARD2_HUMAN antibody
      • JARID2 antibody
      • JMJ antibody
      • Jumonji AT rich interactive domain 2 antibody
      • Jumonji homolog antibody
      • Jumonji like protein antibody
      • Jumonji protein antibody
      • Jumonji/ARID domain containing protein 2 antibody
      • Jumonji/ARID domain-containing protein 2 antibody
      • Protein Jumonji antibody
      see all

    Images

    • Western blot - Anti-Jarid2 antibody (ab184152)
      Western blot - Anti-Jarid2 antibody (ab184152)
      All lanes : Anti-Jarid2 antibody (ab184152) at 1/20000 dilution

      Lane 1 : 293T whole cell lysate
      Lane 2 : Jarid2-transfected 293T whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 138 kDa



      5 % SDS-PAGE

    • Western blot - Anti-Jarid2 antibody (ab184152)
      Western blot - Anti-Jarid2 antibody (ab184152)
      Anti-Jarid2 antibody (ab184152) at 1/1000 dilution + Mouse fetal forebrain lysate at 50 µg

      Predicted band size: 138 kDa



      5 % SDS-PAGE

    • Immunocytochemistry/ Immunofluorescence - Anti-Jarid2 antibody (ab184152)
      Immunocytochemistry/ Immunofluorescence - Anti-Jarid2 antibody (ab184152)

      Immunofluorescent analysis of paraformaldehyde fixed Jarid2 transfected (right) or non-transfected (left) HeLa cells, labeling Jarid2 using ab184152 at 1/500 dilution.  Lower images costained with Hoechst 33342.

    References

    ab184152 has not yet been referenced specifically in any publications.

    Publishing research using ab184152? Please let us know so that we can cite the reference in this datasheet.

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