Overview

  • Product nameAnti-Jarid2 antibody
    See all Jarid2 primary antibodies
  • Description
    Rabbit polyclonal to Jarid2
  • SpecificityThis antibody is specific for Jarid2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide made to an N-terminal portion of the human Jarid2 protein, between residues 1-100

  • Positive control
    • E16 mouse brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab48137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa).

Target

  • FunctionRegulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.
  • Tissue specificityDuring embryogenesis, predominantly expressed in neurons and particularly in dorsal root ganglion cells.
  • Sequence similaritiesContains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
  • DomainThe ARID domain is required to target the PRC2 complex to its target genes.
    The GSGFP motif is required for the interaction with SUZ12.
  • Cellular localizationNucleus. Colocalizes with the PRC2 complex on chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • JARD2 antibody
    • JARD2_HUMAN antibody
    • JARID2 antibody
    • JMJ antibody
    • Jumonji AT rich interactive domain 2 antibody
    • Jumonji homolog antibody
    • Jumonji like protein antibody
    • Jumonji protein antibody
    • Jumonji/ARID domain containing protein 2 antibody
    • Jumonji/ARID domain-containing protein 2 antibody
    • Protein Jumonji antibody
    see all

Anti-Jarid2 antibody images

  • Anti-Jarid2 antibody (ab48137) at 2 µg/ml + E16 mouse brain lysate at 50 µg

    Predicted band size : 139 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,80 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Jarid2 antibody (ab48137)

This product has been referenced in:
  • van Heeringen SJ  et al. Principles of nucleation of H3K27 methylation during embryonic development. Genome Res 24:401-10 (2014). ChIP ; Xenopus tropicalis . Read more (PubMed: 24336765) »
  • Sourvinos G  et al. The downregulation of GFI1 by the EZH2-NDY1/KDM2B-JARID2 axis and by human cytomegalovirus (HCMV) associated factors allows the activation of the HCMV major IE promoter and the transition to productive infection. PLoS Pathog 10:e1004136 (2014). WB ; Human . Read more (PubMed: 24830456) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 800000 cells
Specification HeLa
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 08 2011

I am sorry to hear that you have been experiencing problems with ab48137 in WB. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"