JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab112133)


  • Product nameJC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry)
    See all Mitochondrial Transmembrane Potential kits
  • Tests
    1 x 100 test
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Product overview

    Although JC-1 is widely used in many labs, its poor water solubility causes great inconvenience. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 is developed to be a superior alternative to JC-1 when high dye concentration is desired. Compared to JC-1, JC-10 has much better water solubility. JC-10 is capable of selectively entering into mitochondria, and reversibly changes its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate form). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized. Both colors can be detected using the filters commonly mounted in all flow cytometers. The green emission can be analyzed in fluorescence channel 1 (FL1) and greenish orange emission in channel 2 (FL2). Besides its use in flow cytometry, it can also be used in fluorescence imaging and fluorescence microplate platform.

    ab112133 is based on the detection of the mitochondrial membrane potential changes in cells by the cationic, lipophilic JC-10 dye. In normal cells, JC-10 concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. However, in apoptotic and necrotic cells, JC-10 exists in monomeric form and stains cells green.
    ab112133 is optimized to screen both apoptosis activators and inhibitors using flow cytometry while ab112134 is offered for microplate-based high throughput screening applications.

    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsSuitable for: Flow Cytmore details


  • RelevanceMitochondrial Transmembrane Potential is an important parameter of mitochondrial function used as an indicator of cell death. The collapse of the mitochondrial transmembrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
  • Alternative names
    • mitochondrial membrane potential

Associated products


Our Abpromise guarantee covers the use of ab112133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) images

  • ab112133 was used to measure the effect of FCCP induced mitochondria membrane potential change in Jurkat cells by Flow Cytometry. Jurkat cells were dye loaded with JC-10 dye-loading solution along with DMSO (Top) or 5 µM FCCP (Low) for 10 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-10 were measured with a flow cytometer using FL1 and FL2 channels. Uncompensated data (left column) were compared with compensated data (right column).


References for JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab112133)

This product has been referenced in:
  • Khurana S  et al. Antiapoptotic actions of methyl gallate on neonatal rat cardiac myocytes exposed to H2O2. Oxid Med Cell Longev 2014:657512 (2014). Flow Cyt ; Rat . Read more (PubMed: 24672637) »
  • Liu Z  et al. Potent Half-Sandwich Iridium(III) Anticancer Complexes Containing C(?)N-Chelated and Pyridine Ligands. Organometallics 33:5324-5333 (2014). Read more (PubMed: 25328266) »

See all 2 Publications for this product

Product Wall

This kit is for live cells only.

Based on the data, it looks like the way your customer treated the cells probably is the reason for the problem. It most likely when detaching the cells (trypsinized?), the mitochondria membrane potential changes. If you have to use flow, might try EDT...

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The concentration of CCCP or FCCP required as a positive control will vary based on cell line. Perhaps it would be useful to try 50uM FCCP as this has worked for you in the past.

Thank you for providing that extra information.

I have talked to the lab again, to see if they have any other thoughts on this and they think it is probably due to using the different "gating method", attached please find a copy of ...

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Thank you for providing that information.

I have talked to our supplier about your concerns and they have told me that your results are most likely due to the method of preparing the cells. Physically scraped the cells may damage the cell mem...

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