Overview

  • Product nameAnti-JHDM1D antibody
    See all JHDM1D primary antibodies
  • Description
    Rabbit polyclonal to JHDM1D
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide aa 750-850 conjugated to keyhole limpet haemocyanin.
    Database link: Q6ZMT4

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Hela, Jurkat, HEK293.

Properties

Applications

Our Abpromise guarantee covers the use of ab36044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).

Target

  • FunctionHistone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate.
  • Sequence similaritiesBelongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
    Contains 1 JmjC domain.
    Contains 1 PHD-type zinc finger.
  • DomainThe PHD-type zinc finger mediates the binding to H3K4me3. Binding to H3K4me3 prevents its access to H3K9me2.
    The linker region is a critical determinant of demethylase specificity. It prevents the active site of JmjC to reach the target H3K9me2 when the PHD-type zinc finger binds to H3K4me3, while it favors selectivity toward H3K27me2.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • JHDM1D antibody
    • jmjc containing protein antibody
    • JmjC domain-containing histone demethylation protein 1D antibody
    • jumonji containing protein antibody
    • KDM7_HUMAN antibody
    • kiaa1718 antibody
    • Lysine-specific demethylase 7 antibody
    see all

Anti-JHDM1D antibody images

  • All lanes : Anti-JHDM1D antibody (ab36044) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 107 kDa
    Observed band size : 107 kDa


    Exposure time : 2 minutes

References for Anti-JHDM1D antibody (ab36044)

This product has been referenced in:
  • Huang C  et al. Dual-specificity histone demethylase KIAA1718 (KDM7A) regulates neural differentiation through FGF4. Cell Res 20:154-65 (2010). WB ; Human . Read more (PubMed: 20084082) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"