Overview

  • Product nameAnti-JIP1 antibody
    See all JIP1 primary antibodies
  • Description
    Rabbit polyclonal to JIP1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Mouse) (N terminal)

  • Positive control
    • Mouse Intestine. Rat kidney. Jurkat cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 0.1% BSA, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab24449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). A band of 110 kDa can also be observed in human samples.

Target

  • RelevanceThe JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module and is required for JNK activation in response to excitotoxic stress. Cytoplasmic JIP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. It may also participate in ApoER2-specific reelin signaling and directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. It appears to have a role in cell signaling in mature and developing nerve terminals and may function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. It functions as an anti-apoptotic protein whose level seems to influence the beta-cell death or survival response.
  • Cellular localizationCytoplasmic. Accumulates in cell surface projections. Under certain stress conditions, translocates to the perinuclear region of neurons. In insulin-secreting cells, detected in both the cytoplasm and nucleus
  • Database links
  • Alternative names
    • C jun amino terminal kinase interacting protein 1 antibody
    • IB 1 antibody
    • IB1 antibody
    • Islet brain 1 antibody
    • JIP 1 antibody
    • JNK interacting protein 1 antibody
    • JNK MAP kinase scaffold protein 1 antibody
    • MAPK8IP 1 antibody
    • MAPK8IP1 antibody
    • MAPK8IP1 mitogen-activated protein kinase 8 interacting protein 1 antibody
    • Mitogen activated protein kinase 8 interacting protein 1 antibody
    • PRKM8 antibody
    • PRKM8 interacting protein antibody
    • PRKM8IP antibody
    see all

Anti-JIP1 antibody images

  • All lanes : Anti-JIP1 antibody (ab24449)

    Lane 1 : Rat kidney
    Lane 2 : Mouse Intestine
    Lane 3 : Jurkat Cell lysate


    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Additional bands at : 110 kDa (possible isoform).
  • ICC/IF image of ab24449 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24449, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-JIP1 antibody (ab24449)

ab24449 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. To our knowledge, the antibody has yet to be tested in immunoprecipitation. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, we encourage you to s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"